D34 Cell Line

D34 Cell Line is derived from a leukoplakia biopsy. The cell line was notable in its proliferative capacity, considered immortal after having completed more than 100 PDs (population doublings ) when maintained on a feeder layer of irradiated 3T3 fibroblasts. D34 was categorised as having a moderate dysplasia pathology.

Contributors

Inventor Institute
Paul Harrison Cancer Research UK, Glasgow: The Beatson Institute
Cat. #: 152857
Tool sub type: 10XUAS IVS p10
Unit size: 1×10^6 cells / vial
Organism: Human
Tissue: Tongue
Gender: Male
Product description: D34 Cell Line is derived from a leukoplakia biopsy. The cell line was notable in its proliferative capacity, considered immortal after having completed more than 100 PDs (population doublings ) when maintained on a feeder layer of irradiated 3T3 fibroblasts. D34 was categorised as having a moderate dysplasia pathology.
Gender: Male
CRISPR: Yes
Conditional: No
Production details: Biopsies were trypsinized and cultured until a growing population of cells was obtained in a 9-cm plate and then passaged once to give a stock culture that was frozen. All cells were maintained on irradiated 3T3 feeders, in 10H medium. The 3T3 feeder layer was removed by treatment with 0.02% EDTA prior to RNA and protein extraction.
Cellosaurus ID: CVCL_HF98
Disease: Cancer
Format: Frozen
Shipping conditions: Dry ice
Growth medium: As per Cancer Res. 1997 Sep 15;57(18):3886-9. All cells were maintained on irradiated 3T3 feeders, in 10H medium (DMEM plus 10% FCS without added growth factors except hydrocortisone).
Mycoplasma free: Yes
References:

McGregor et al. 2002. Cancer Res. 62(16):4757-66. PMID: 12183435.

Molecular changes associated with oral dysplasia progression and acquisition of immortality: potential for its reversal by 5-azacytidine.

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