CMT 93/69 Cell Line

The CMT 93/69 Cell Line is a derivative of CMT93. Access the cell line through the non-profit CancerTools.org, part of Cancer Research UK

Contributors

Institute
Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Cat. #: 152753
Unit size: 1×10^6 cells / vial
Cancer types: Digestive / Gastrointestinal cancer
Research Fields: Cancer;Drug development
Organism: Mouse
Model: Tumour line
Growth properties: Cells grow in coherent clumps.
Cancer Types In Detail: Mouse C57BL/1CRF rectum carcinoma
Product description: The CMT 93/69 Cell Line is a derivative of CMT93. The CMT 93 has been isolated from a 19 month old male mouse (C57BL/1CRF) which had received an i.p. injection of MAMA each week for 18 months. 4th in vivo passage – source of explant culture. Cells form acini and junctional complexes and produce large tumours in nude mice (6 x 1,000,000 cells inoculum) after 1 month.
Conditional: Yes
Cellosaurus ID: CVCL_4147
disease: Cancer
Format: Frozen
Shipping conditions: Dry ice
Growth medium: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4×10,000 cells/cm?‹Â› using 0.25% trypsin/EDTA; 5% CO2; 37?‚° C. Culture Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)
References:

Franks et al. 1978. J Pathol. 124(1):35-8. PMID: 722371.

A cell line from an induced carcinoma of mouse rectum.

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