CMT 93 Cell Line

The CMT 93 cell line has been isolated from a 19 month old male mouse (C57BL/1CRF) which had received an i.p. injection of MAMA each week for 18 months.

Contributors

Institute
Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Cat. #: 152752
Unit size: 1×10^6 cells / vial
Cancer types: Digestive / Gastrointestinal cancer
Research Fields: Cancer;Drug development
Organism: Mouse
Model: Tumour line
Growth properties: Adherent
Cancer Types In Detail: Mouse rectum carcinoma
Product description: The CMT 93 cell line has been isolated from a 19 month old male mouse (C57BL/1CRF) which had received an i.p. injection of MAMA each week for 18 months. 4th in vivo passage – source of explant culture. Cells form acini and junctional complexes and produce large tumours in nude mice (6 x 1,000,000 cells inoculum) after 1 month.
Conditional: Yes
Cellosaurus ID: CVCL_1986
disease: Cancer
Format: Frozen
Shipping conditions: Dry ice
Growth medium: Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-3×10,000 cells/cm?‹Â› using 0.25% trypsin; 5% CO2; 37?‚°C. Cells grow in clumps. Culture Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
References:

Franks et al. 1978. J Pathol. 124(1):35-8. PMID: 722371.

A cell line from an induced carcinoma of mouse rectum.

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