CHO ICAM-1Fc Cell Line

CHO ICAM-1Fc cells secrete a dimeric human ICAM-1Fc protein. ICAM-1 (CD54) is a major ligand for leukocyte CD11/CD18 integrins and used in adhesion assays to evaluate integrin activity.

Contributors

Inventor Institute
Nancy Hogg Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Cat. #: 152756
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Research Fields: Cell biology;Immunology
Organism: Hamster
Tissue: Ovary
Model: Transgenic
Product description: CHO ICAM-1Fc cells secrete a dimeric human ICAM-1Fc protein. ICAM-1 (CD54) is a major ligand for leukocyte CD11/CD18 integrins and used in adhesion assays to evaluate integrin activity.
Conditional: No
Production details: The chimeric wild-type ICAM-1Fc protein consists of the five extracellular domains of ICAM-1 joined to the hinge, CH2 and CH3 domains of IgG1. CHO cells were transfected using DEAE-dextran and the secreted proteins were collected in serum-free medium for 4 days. The proteins were purified on a Protein Aâ?‚€?‚“Sepharose affinity column using standard methods.
Cellosaurus ID: CVCL_AS85
Parental cell line: Chinese Hamster Ovary (CHO)
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Recommended controls: Chinese Hamster Ovary (CHO)
Mycoplasma free: Yes
Biosafety level: 1
References:

Stanley et al. 2008. EMBO J. 27(1):62-75. PMID: 18079697.

Intermediate-affinity LFA-1 binds alpha-actinin-1 to control migration at the leading edge of the T cell.

Smith et al. 2005. J Cell Biol. 170(1):141-51. PMID: 15983060.

A talin-dependent LFA-1 focal zone is formed by rapidly migrating T lymphocytes.

Smith et al. 2003. J Cell Sci. 116(Pt 15):3123-33. PMID: 12799414.

LFA-1-induced T cell migration on ICAM-1 involves regulation of MLCK-mediated attachment and ROCK-dependent deta…

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