#152756

CHO ICAM-1Fc Cell Line

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Cat. #152756

CHO ICAM-1Fc Cell Line

Cat. #: 152756

Sub-type: Continuous

Unit size: 1x10^6 cells / vial

Availability: 3-5 days

Organism: Hamster

Tissue: Ovary

Model: Transgenic

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Nancy Hogg

Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details
Target Details
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: CHO ICAM-1Fc Cell Line
  • Research fields: Cell biology;Immunology
  • Tool sub type: Continuous
  • Parental cell: Chinese Hamster Ovary (CHO)
  • Organism: Hamster
  • Tissue: Ovary
  • Model: Transgenic
  • Conditional: No
  • Description: CHO ICAM-1Fc cells secrete a dimeric human ICAM-1Fc protein. ICAM-1 (CD54) is a major ligand for leukocyte CD11/CD18 integrins and used in adhesion assays to evaluate integrin activity.
  • Production details: The chimeric wild-type ICAM-1Fc protein consists of the five extracellular domains of ICAM-1 joined to the hinge, CH2 and CH3 domains of IgG1. CHO cells were transfected using DEAE-dextran and the secreted proteins were collected in serum-free medium for 4 days. The proteins were purified on a Protein Aâ?‚€?‚“Sepharose affinity column using standard methods.
  • Biosafety level: 1
  • Recommended controls: Chinese Hamster Ovary (CHO)
  • Cellosaurus id: CVCL_AS85

Target Details

  • Target: Human ICAM-1Fc chimeric protein (five extracellular domains of ICAM-1 plus hinge, CH2 and CH3 domains of IgG1)

Handling

  • Format: Frozen
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice
  • Storage conditions: Liquid Nitrogen
  • Mycoplasma free: Yes

References

  • Stanley et al. 2008. EMBO J. 27(1):62-75. PMID: 18079697.
  • Intermediate-affinity LFA-1 binds alpha-actinin-1 to control migration at the leading edge of the T cell.
  • Smith et al. 2005. J Cell Biol. 170(1):141-51. PMID: 15983060.
  • A talin-dependent LFA-1 focal zone is formed by rapidly migrating T lymphocytes.
  • Smith et al. 2003. J Cell Sci. 116(Pt 15):3123-33. PMID: 12799414.
  • LFA-1-induced T cell migration on ICAM-1 involves regulation of MLCK-mediated attachment and ROCK-dependent deta...