Cat. #161906
Calu-3 Cell line
Cat. #: 161906
Availability: 8-10 weeks
Organism: Human
Tissue: Lung
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Jorgen Fogh, Germain Trempe
Institute: Memorial Sloan-Kettering Cancer Center (MSK)
Primary Citation: Fogh J et al.1977.Journal of the National Cancer Institute. 59: 221-226.PMID: 327080.
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Calu-3 Cell line
- Cancer: Adenocarcinoma
- Organism: Human
- Gender: Male
- Tissue: Lung
- Donor: 25-year-old, White, male patient with lung adenocarcinoma who received prior therapy with cytoxan, bleomycin, and adriamycin.
- Morphology: Epithelial
- Growth properties: Adherent
- Products or characteristics of interest: Isoenzymes: AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 1-2; Me-2, 1; PGM1, 1; PGM3, 1-2; ES-D, 1; G6PD, B; GLO-I, 1-2; Me-2, 1-2; PGM1, 1-2; PGM3, 1
- Description: Calu-3 is a non-small-cell lung cancer cell line that grows in adherent culture and displays epithelial morphology. These cells have constitutively active ErbB2/Her2 due to amplification of the ERBB2 gene. They express wildtype EGFR and mutant K-Ras (G13D). In addition, they harbour mutations in TP53 and CDKN2A genes. The Calu-3 cells are sensitive to erlotinib (EGFR tyrosine kinase inhibitor) and cetuximab (a monoclonal antibody that blocks ligand binding to EGFR and prevents downstream signalling), two commonly used drugs targeting ErbB receptors. These cells are capable of forming tumours in immunocompromised mice.
- Application: 3D cell culture; High-throughput screening; Toxicology studies; Bioproduction; This cell line is a suitable transfection host and can be used for the propagation of SARS-CoV-2 in vitro.
- Biosafety level: 1
Target Details
- Target: Antigen Expression: Blood Type A; Rh+; HLA A2, A9, B13, B17
Applications
- Application: 3D cell culture; High-throughput screening; Toxicology studies; Bioproduction; This cell line is a suitable transfection host and can be used for the propagation of SARS-CoV-2 in vitro.
Handling
- Growth medium: EMEM suplemented with 10% FBS.
- Temperature: 37° C
- Atmosphere: 95% Air, 5% CO2
- Shipping conditions: Dry Ice
- Storage medium: Culture medium 95%; DMSO, 5%
- Storage conditions: Vapor phase of liquid nitrogen. Storage at -70° C will result in loss of viability.
- Subculture routine: 1. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. 2. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
- Str profiling: Amelogenin: X;CSF1PO: 11,12;D13S317: 12;D16S539: 12,14;D5S818: 11;D7S820: 10,11;TH01: 6,9.3;TPOX: 8;vWA: 16,17;D3S1358: 15,18;D21S11: 28,30;D18S51: 14,17;Penta_E: 5,21;Penta_D: 9,16;D8S1179: 11,15;FGA: 25;D19S433: 12,13;D2S1338: 19
References
- Fogh J et al.1977.Journal of the National Cancer Institute. 59: 221-226.PMID: 327080.
- Cavazzoni A et al. 2007. Cancer Letters. 246: 69-81. PMID: 16616810.
- Blanco R et al. 2009. Human Mutation. 30: 1199–1206. PMID: 19472407.
