C32 Colorectal adenocarcinoma Cell Line

This C32 cell line was established from a 66-year old female patient with a well differentiated adenocarcinoma of the colon classified as Dukes’ stage C.

Contributors

Inventor Institute
Walter Bodmer University of Oxford
Cat. #: 151756
Unit size: 1×10^6 cells / vial
Research Fields: Cancer;Drug development
Organism: Human
Tissue: Colon
Model: Tumour line
Alternate name: C32 colon
Product description: The C32 cell line was established from a 66-year old female patient with a well differentiated adenocarcinoma of the colon classified as Dukes' stage C. C32 harbours mutations in NRAS, SMAD4 and APC.
Conditional: Yes
Cellosaurus ID: CVCL_5246
Format: Frozen
Shipping conditions: Dry ice
Growth medium: Iscove's Modified Dulbecco's Medium, + 10% Foetal Bovine Serum (FBS) + 2 mM Glutamine.
Subculture routine: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4×10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C. C32 cells grow very slowly; following resuscitation or subculture the cells take at least 48 hours to re-attach. Cells should be left without disturbance during this time to facilitate adhesion. Centrifugation of the cells (100g x 5 min) at resuscitation to remove DMSO improves the establishment of a viable culture. Once attached, the cells grow in discrete islands and use of trypsin or trypsin/EDTA to subculture the cells (even without knocking the flask) yields large clumps. Further disaggregation may be achieved by repeatedly pipetting the cells.
References:

Browning et al. 1993. Proc Natl Acad Sci U S A. 90(7):2842-5. PMID: 8464898.

Images

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C32 Cell Line - growth profile. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC), UK.

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