C125PM Colorectal Cell Line

C125PM is a human colorectal adenocarcinoma cell line.

Contributors

Inventor Institute
Walter Bodmer Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Cat. #: 151763
Tool sub type: 10XUAS IVS p10
Unit size: 1×10^6 cells / vial
Organism: Human
Tissue: Colon
Gender: Male
Model: Tumour line
Growth properties: Adherent
Cancer Types In Detail: Adenocarcinoma;Adenocarcinoma
Alternate name: C125PM; C125
Product description: C125PM is a human colorectal adenocarcinoma cell line.
Gender: Male
CRISPR: Yes
Conditional: Yes
Cellosaurus ID: CVCL_8175
disease: Cancer;Cancer
Format: Frozen
Shipping conditions: Dry ice
Growth medium: Iscove's Modified Dulbecco's Medium, + 10% Foetal Bovine Serum (FBS) + 2 mM Glutamine.
Subculture routine: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4×10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C. C125PM cells grow slowly (see growth curve); following resuscitation or subculture the cells take at least 48 hours to re-attach. Cells should be left without disturbance during this time to facilitate adhesion. Centrifugation of the cells (100g x 5 min) at resuscitation to remove DMSO improves the establishment of a viable culture. Once attached, the cells grow in discrete islands and use of trypsin or trypsin/EDTA to subculture the cells (even without knocking the flask) yields large clumps. Further disaggregation may be achieved by repeatedly pipetting the cells.
Mycoplasma free: Yes
References:

Mariella et al. 2024. Mol Oncol. 18(6):1460-1485. PMID: 38468448.

Frejno et al. 2017. Mol Syst Biol. 13(11):951. PMID: 29101300.

Medico et al. 2015. Nat Commun. 30:7002. PMID: 25926053.

Mouradov et al. 2014. Cancer Res. 74(12):3238-3247. PMID: 24755471.

Schlicker et al. 2012. BMC Med Genomics. 5:66. PMID: 23272949.

Janakiraman et al. 2010. Cancer Res. 70(14):5901-5911. PMID: 20570890.

Emaduddin et al. 2008. Proc Natl Acad Sci U S A. 105(7):2358-2362. PMID: 18258742.

Liu et al. 2006. Proc Natl Acad Sci U S A. 103(4):976-981. PMID: 16418264.

Rowan et al. 2000. Proc Natl Acad Sci U S A. 97(7):3352-3357. PMID: 10737795.

Images

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C125PM cell line growth profile. Medium changed at 70 hours. Image courtesy of European Collection of Authenticated Cell Cultures (ECACC).

Citations And Reviews

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