BMI-1 5 Cell Line

Primary Human bronchial epithelial cells when grown in vitro have a limited lifespan and begin to deviate both in phenotype and morphology, losing the plasticity required around passage 4 or 5, for air-liquid interface (ALI) differentiation. These Human bronchial epithelial cells expressing BMI-1 retain both viability and differentiation potential of wild-type human bronchial epithelium while […]

Contributors

Inventor Institute
Ian Sayers University of Nottingham
Cat. #: 153585
Tool sub type: Primary
Unit size: 1×10^6 cells / vial
Research Fields: Cell biology;Cell signaling and signal transduction;Drug development;Genetics
Organism: Human
Tissue: Lung
Gender: Female
Model: Extended Lifespan
Alternate name: Polycomb complex protein BMI-1, polycomb group RING finger protein 4; PCGF4; RING finger protein 51; RNF51; B cell-specific Moloney murine leukemia virus integration site 1; BMI-1; FLVI2/PCGF4; Mo-MLV; Moloney murine leukemia virus; NHBEC; Normal Human Bronchial Epithelial Cells; pLVX-BMI-1 Cell Line: COPD, ALI; airâ?‚€?‚“liquid interface
Product description: Primary Human bronchial epithelial cells when grown in vitro have a limited lifespan and begin to deviate both in phenotype and morphology, losing the plasticity required around passage 4 or 5, for air-liquid interface (ALI) differentiation. These Human bronchial epithelial cells expressing BMI-1 retain both viability and differentiation potential of wild-type human bronchial epithelium while importantly not demonstrating changes in cell karyotype.B lymphoma Moloney murine leukemia virus insertion region 1 homolog (BMI-1) is an oncogene which functions by regulating P16 and P19 cell cycle inhibitor genes and is also associated with erythroplakia and tongue cancer. BMI-1 is thought to repress, p16(Ink4a), a cyclin-dependent kinase inhibitor and tumor suppressor that induces cell cycle arrest at the Gap 1 phase. BMI-1 can therefore be used to delay cell senescence. The airway epithelium is a critical interface acting as a barrier to potential pathogens and extraneous particles, assisting in regulation of host defense mechanisms like the inflammatory response.
Gender: Female
Production details: Passage 2 heterogeneous cells were plated in a 6-well plate at 5 x 10 4 cells per well and grown overnight. Media was replaced with 800 ul of media with 2 ug/ml of polybrene and 6.25 uL of lentivirus pLVX-BmI-1 to give >90% transfection. The plates were incubated at 37Â?‚°C for 6.5 h with gentle rocking and the media replaced after 2 hours. After 72 h, selection media containing 300 ng/mL puromycin was added and cells with antibiotic resistance after 6 days of selection were considered to…
Additional notes: Cells were fixed for immunostaining, histology sectioning, and scanning electron microscopy after 28 days at ALI.
disease: Respiratory disease
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Growth medium: Normal human bronchial epithelial cells were grown in a growth factor-supplemented medium and differentiated at ALI in bronchial epithelial differentiation medium.
Mycoplasma free: Yes
Biosafety level: 1
References:

Torr et al. 2016. Physiol Rep. 4(16):. PMID: 27558999.

Expression of polycomb protein BMI-1 maintains the plasticity of basal bronchial epithelial cells.

Related Tools for Cell lines

Cat. # Tool Name
151450 JIM3 Cell Line Key Info View Tool
151479 HCT 116 p300 KO [D10] Cell Line Key Info View Tool
151480 HEK293A EGFP-LC3 Cell Line Key Info View Tool
151576 MEF RAF1 KO Cell Line Key Info View Tool
151626 A375P beta6-puro Cell Line Key Info View Tool

Related News

UM-UC-10 Cell Line. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC), UK.
Cell Lines

Popular cell lines now available only from CancerTools.org

Read More
Cell Lines

New cell lines: murine melanocyte and melanoblast cell lines from Dr. Elena Sviderskaya

Read More
Image Reference: 2x PTEN -/- mouse prostate cancer cell lines (MuCap system). Histology staining of syngraft tumours (Van Duijn et al., 2018).
Cell Lines

38 new prostate cancer cell lines

Read More

Tool enquiry

Please ensure you use your organisation email address rather than personal where possible, as this helps us locate your organisation in our system faster.

Please note we may take up to three days to respond to your enquiry.

CancerTools.org uses the contact information provided to respond to you about our research tools and service. For more information please review our privacy policy.