Cisplatin resistant (CisR) Non-Small Cell Lung Cancer Cell lines
| Inventor | Institute |
|---|---|
| Martin P. Barr | Trinity College Dublin |
| SKU: | 162205 |
|---|---|
| Product description: | Cisplatin resistant (CisR) Non-Small Cell Lung Cancer Cell lines |
| Alternate name: | A549 Chemo-resistant cell line |
| Gender: | Male |
| CRISPR: | Yes |
| Conditional: | Yes |
| Parental cell line: | A549 |
| Cat. #: | 162205 |
|---|---|
| Tool sub type: | 10XUAS IVS p10 |
| Cancer types: | Lung cancer |
| Organism: | Human |
| Tissue: | Lung |
| Gender: | Male |
| Model: | Therapy Resistance / Drug (Cisplatin) / Extrinsic |
| Morphology: | Epithelial |
| Growth properties: | Adherent |
| Primary citation: | Barr MP, et al. 2013. PLoS One. 8(1):e54193. PMID: 23349823 |
| Format: | Frozen |
|---|---|
| Storage conditions: | Liquid Nitrogen |
| Shipping conditions: | Dry ice |
| Growth medium: | Hams-F12 media supplemented with 10% FBS, 1% Penicillin/Streptomycin, 4mM L-Glutamine (final concentration) |
| Subculture routine: | Subculture cells using trypsin-EDTA. Neutralise trypsin in supplemented cell culture media and pellet cells by centrifugation at 1300 rpm for 3 min. Discard supernatant to waste and resuspend the cell pellet in 1 mL of supplmented cell culture media. Transfer cell content to a T75 cm2 vented culture flask and incubate until further use. |
| Atmosphere: | 5% CO2 |
| Mycoplasma free: | Yes |
| References: |
Barr MP, et al. 2013. PLoS One. 8(1):e54193. PMID: 23349824 |
|---|
Please note we may take up to three days to respond to your enquiry.
CancerTools.org uses the contact information provided to respond to you about our research tools and service. For more information please review our privacy policy.