Cat. #154462
16E6/E7-HSK cell line
Cat. #: 154462
Unit size: 1x10^6 cells / vial
Availability: 8-10 weeks
Organism: Human
Tissue: Skin
Disease: Cancer
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Aloysius Klingelhutz
Institute: The University of Iowa
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: 16E6/E7-HSK cell line
- Research fields: Cancer
- Parental cell: Keratinocytes isolated from punch biopsies of the skin from the buttocks
- Organism: Human
- Tissue: Skin
- Disease: Cancer
- Growth properties: Upregulation of telomerase and greatly extended lifespan (greater than 5 times the lifespan of untransduced keratinocytes) with no apparent crisis or slow down in growth. Despite upregulation of telomerase, E6/E7 transduced keratinocytes exhibited significant telomere shortening with passaging in culture
- Conditional: No
- Description: Expresses skin cell markers; proinflammatory. Tissue is adult skin cells.
- Production details: Cells were transduced with HPV-16 E6/E7 LXSN replication defective retrovirus and selected in G418
- Biosafety level: 1
Target Details
- Target: Used as an immortal adult human skin keratinocyte line for pathogenesis and inflammation studies
Handling
- Format: Frozen
- Growth medium: Keratinocyte Serum Free Medium (K-SFM) (Gibco™, 17005042) supplemented with 0.16 ng/mL EGF and 25 ug/mL BPE. Do not keep media for more than 1 month past adding supplements. Do not freeze and thaw media. Change media every other day. The cell line will not do well if they are left over the weekend without a media change or if they are left to grow to greater than 95% confluency.
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
- Storage conditions: Liquid Nitrogen
- Mycoplasma free: Yes
References
- Gourronc et al. 2010. Exp Dermatol. 19(3):279-88. PMID: 19558498.
