Cat. #161679
Interstitial Fluid-modeled Medium
Cat. #: 161679
Sub-type: Cell culture media
Application: Standard culture / maintenance of cells, and during infection with the parasitic bacterium Coxiella burnetii (isolate: RSA439).
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Anders Omsland
Institute: Washington State University
Primary Citation: S.E.Sanchez Infect Immun. 2021 Nov 16,89(12):e0013521. PMID: 34491791
Tool Details
*FOR RESEARCH USE ONLY
- Tool name: Interstitial Fluid-modeled Medium
- Alternate name: IFmM
- Tool sub type: Cell culture media
- Purpose: Culture medium for eukaryotic cells based on the chemical composition of interstitial fluid
- Description: Most cell culture media were designed based on easy of use and the ability to support maximal growth of continuous cell lines but do not necessarily reflect physiological conditions. For example, the concentration of glucose in some cell culture media is considerably higher than what one would be considerd normal in humans. IFmM is a cell culture medium based on the chemical composition of interstitial fluid (IF). IF is essentially a filtrate of plasma and the conduit of nutrients for cells/tissues outside the vasculature
- Cell lines validated: J774A.1 (ATCC: TIB-67), THP-1 (ATCC: TIB-202), JEG-3 (ATCC: HTB-36), and VERO (ATCC: CCL-81).
- Formulation: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8594591/table/T1/
- Application: Standard culture / maintenance of cells, and during infection with the parasitic bacterium Coxiella burnetii (isolate: RSA439).
Handling
- Storage conditions: Store refrigerated. Stability / shelf-life not tested but expected to be similar to commercial cell culture media.
- Shipping conditions: Same as other culture media: Ship at ambient temp then store at 4 degrees upon delivery.
Application Details
- Application: Standard culture / maintenance of cells, and during infection with the parasitic bacterium Coxiella burnetii (isolate: RSA439).
- Application notes: IFmM should be supplemented with FBS and/or antibiotics based on the culture requirement(s) for any cell line in question
References
- S.E.Sanchez Infect Immun. 2021 Nov 16,89(12):e0013521. PMID: 34491791
