The transparency of the juvenile zebrafish and its genetic advantages make it an attractive model for the study of intestinal differentiation and renewal. This antibody labels the secretory cells of…
| Inventor | Institute |
|---|---|
| Cecile Wright-Crosnier ; Julian Lewis ; Linda Ariza-McNaughton ; Neil Vargesson | Cancer Research UK, London Research Institute: Lincoln's Inn Fields |
| Cat. #: | 151517 |
|---|---|
| Tool sub type: | Primary antibody |
| Unit size: | 100 ug |
| Cancer types: | Colorectal cancer |
| Research Fields: | Cell biology;Cell signaling and signal transduction;Developmental biology;Genetics;Stem cell biology;Tags and cell markers |
| Application: | IF ; WB |
| Target: | Zebrafish gut secretory cell epitopes |
| Reactivity: | Zebrafish |
| Clone: | FIS 4B7/2 |
| Host: | Mouse |
| Class: | Monoclonal |
| Product description: | The transparency of the juvenile zebrafish and its genetic advantages make it an attractive model for the study of intestinal differentiation and renewal. This antibody labels the secretory cells of the zebrafish intestinal epithelium, both mucous and enteroendocrine, corresponding to the class of cells that are lost in MathI mouse mutants (Yang et al, (2001) Science 294: 2155-2158) |
|---|---|
| Conjugation: | Unconjugated |
| Isotype: | IgG1 |
| Molecular weight: | 35 kDa |
| Immunogen: | Lysate of fish intestine |
| Myeloma used: | Sp2/0-Ag14 |
| Target background: | The transparency of the juvenile zebrafish and its genetic advantages make it an attractive model for the study of intestinal differentiation and renewal. This antibody labels the secretory cells of the zebrafish intestinal epithelium, both mucous and enteroendocrine, corresponding to the class of cells that are lost in MathI mouse mutants (Yang et al, (2001) Science 294: 2155-2158) |
|---|
| Format: | Liquid |
|---|---|
| Concentration: | 0.9-1.1 mg/ml |
| Storage buffer: | PBS with 0.02% azide |
| Storage conditions: | -15° C to -25° C |
| Shipping conditions: | Dry ice |
| References: |
Matthews et al. 2008. Dev Dyn. 237(1):124-31. PMID: 18095340. Transcription factor onecut3 regulates intrahepatic biliary development in zebrafish. Dong et al. 2007. Nat Genet. 39(3):397-402. PMID: 17259985. Fgf10 regulates hepatopancreatic ductal system patterning and differentiation. Bates et al. 2006. Dev Biol. 297(2):374-86. PMID: 16781702. Distinct signals from the microbiota promote different aspects of zebrafish gut differentiation. Crosnier et al. 2005. Development. 132(5):1093-104. PMID: 15689380. Delta-Notch signalling controls commitment to a secretory fate in the zebrafish intestine. |
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