STRO-1 can be used to positively select for CFU-F, thereby facilitating further studies of stromal elements. STRO-1 ihas recently been shown to recognize an endothelial antigen and is also expressed in many carcinoma cell lines. STRO-I does not bind to hematopoietic progenitor cells.
| Inventor | Institute |
|---|---|
| Beverly TorokStorb | Fred Hutchinson Cancer Center |
| Cat. #: | 161650 |
|---|---|
| Tool sub type: | Primary antibody |
| Research Fields: | Immunology;Stem cell biology |
| Application: | ELISA, FACS, FFPE, Function Blocking, Immunofluorescence, Immunohistochemistry |
| Target: | STRO-1 , Mesenchymal stem cell marker |
| Reactivity: | Human, Mouse, Primate |
| Clone: | STRO1 |
| Host: | Mouse |
| Class: | Monoclonal |
| Primary citation: | Simmons et al. 1991.Blood.78(1)55-62. PMID: 2070060 |
| Product description: | Murine IgM monoclonal antibody STRO-1 identifies a cell surface antigen expressed by stromal elements in human bone marrow (BM). STRO-1 binds to approximately 10% of BM mononuclear cells, greater than 95% of which are nucleated erythroid precursors, but does not react with committed progenitor cells (colony-forming unit granulocyte-macrophage [CFU-GM], erythroid bursts [BFU-E], and mixed colonies [CFU-Mix]). STRO-I binds to BM stromal elements with the capacity to transfer the hematopoieticmicroenvironment in vitro. |
|---|---|
| Isotype: | IgM |
| Immunogen: | CD34 positive (CD34+) Bone Marrow (BM) cells |
| Target background: | STRO-1 binds to human bone marrow(BM) fibroblast-like cells and to various nonhematopoietic cellular components of the adherent cell layer in long-term BM cultures (LTBMC). STRO-1 antibody can also be used to isolate stromal precursors in freshly aspirated BM cell suspensions and has the capacity to transfer the hematopoietic microenvironment in vitro. |
|---|
| Storage conditions: | For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing. |
|---|---|
| Shipping conditions: | Dry ice |
| References: |
Simmons et al. 1991.Blood.78(1)55-62. PMID: 2070060 |
|---|
| Cat. # | Tool Name | |||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 151030 | Anti-Progesterone [11P14] |
Key Info
Anti-Progesterone [11P14]
|
View Tool | |||||||||||||||||||
| 151015 | Anti-CyclinA [E70.1] |
Key Info
Anti-CyclinA [E70.1]
|
View Tool | |||||||||||||||||||
| 151028 | Anti-Cytochrome P450 2C2, 2B1/2 [h7] |
Key Info
Anti-Cytochrome P450 2C2, 2B1/2 [h7]
|
View Tool | |||||||||||||||||||
| 151040 | Anti-CD11 & CD18 [24] |
Key Info
Anti-CD11 & CD18 [24]
|
View Tool | |||||||||||||||||||
| 151018 | Anti-Mos [R38.1] |
Key Info
Anti-Mos [R38.1]
|
View Tool | |||||||||||||||||||
Please note we may take up to three days to respond to your enquiry.
CancerTools.org uses the contact information provided to respond to you about our research tools and service. For more information please review our privacy policy.