Anti-SIGLEC3 [CD33 3D6]

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: Anti-SIGLEC3 [CD33 3D6]
• Alternate name: Sialic Acid Binding Ig Like Lectin 12; SIGLECL1; SLG 3; Siglec-12; Siglec-L1; S2V
• Research fields: Cancer;Cell biology;Immunology;Stem cell biology
• Clone: CD33 3D6
• Tool sub type: Primary antibody
• Class: Monoclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Host: Mouse
• Application: ELISA
• Description: The siglecs are a family of membrane bound lectins (of the immunoglobulin superfamily) that bind sialic acid and mediate cell-cell interactions. Family members include sialoadhesin, CD22 and CD33. CD33 is found on granulocyte and macrophage precursors in the bone marrow, but not on pluripotent stem cells. CD33 is also expressed on, and a useful marker for, peripheral monocytes. CD33 is also useful for distinguishing myelogenous leukaemia cells from lymphoid or erythroid leukaemias.
• Immunogen: CD33-Fc recombinant protein
• Isotype: IgG1
• Myeloma used: Sp2/0-Ag14
• Recommended controls: CD33 transfected COS cells

Target Details

• Target: SIGLEC3 (CD33)
• Tissue cell line specificity: CD33 transfected COS cells
• Target background: The siglecs are a family of membrane bound lectins (of the immunoglobulin superfamily) that bind sialic acid and mediate cell-cell interactions. Family members include sialoadhesin, CD22 and CD33. CD33 is found on granulocyte and macrophage precursors in the bone marrow, but not on pluripotent stem cells. CD33 is also expressed on, and a useful marker for, peripheral monocytes. CD33 is also useful for distinguishing myelogenous leukaemia cells from lymphoid or erythroid leukaemias.

Applications

• Application: ELISA

Handling

• Format: Liquid
• Concentration: 1 mg/ml
• Unit size: 100 ug
• Storage buffer: PBS with 0.02% azide
• Storage conditions: -15° C to -25° C
• Shipping conditions: Shipping at 4° C

References

• Taylor et al. 1999. J Biol Chem. 274(17):11505-12. PMID: 10206955.
• The myeloid-specific sialic acid-binding receptor, CD33, associates with the protein-tyrosine phosphatases, SHP-1 and SHP-2.
• Freeman et al. 1995. Blood. 85(8):2005-12. PMID: 7718872.
• Characterization of CD33 as a new member of the sialoadhesin family of cellular interaction molecules.