Anti-RSV Phosphoprotein VP32 [3-5-18]

This antibody reacts with the phosphoprotein from both human RS virus subgroups A and B as well as the phosphoprotein of bovine RS virus.

Contributors

Inventor Institute
Ayham Alnabulsi Vertebrate Antibodies Limited
Cat. #: 151850
Unit size: 100 ug
Research Fields: Microbiology
Application: ELISA ; IF ; WB
Target: Human Respiratory Syncytial (RS) virus Phosphoprotein, VP32
Reactivity: Virus
Host: Mouse
Class: Monoclonal
Product description: This antibody reacts with the phosphoprotein from both human RS virus subgroups A and B as well as the phosphoprotein of bovine RS virus. The antibody is not reactive with the phosphoprotein of other member of Pneumovirus genus (specifically, pneumonia virus of mice). On the basis of the reactivity of this antibody and the 4-14 antibody (cat number: 151851) against the VP32 protein (by immunoblot and immunofluorescence) the existence of two antigenic types of human RS virus was identified (Gimenez et al., 1986). The phosphoprotein is located within the virion nucleocapsid. The phosphoprotein of RS virus subgroup A is slightly larger than the phosphoprotein of RS virus subgroup B. This difference in molecular weight was demonstrated by immunoblot using this antibody (Gimenez et al., 1986). This antibody is useful for the diagnosis of RS virus infection since the phosphoprotein is abundant which makes the detection assays using this antibody highly sensitive.
Conjugation: Unconjugated
Isotype: IgG1 kappa
Immunogen: Gradient-purified RSN-2 virus (subgroup B) treated with 0.1% SDS at 100°C for 2 mins.
Myeloma used: P3X63Ag8.653
Target background: This antibody reacts with the phosphoprotein from both human RS virus subgroups A and B as well as the phosphoprotein of bovine RS virus. The antibody is not reactive with the phosphoprotein of other member of Pneumovirus genus (specifically, pneumonia virus of mice). On the basis of the reactivity of this antibody and the 4-14 antibody (cat number: 151851) against the VP32 protein (by immunoblot and immunofluorescence) the existence of two antigenic types of human RS virus was identified (Gimenez et al., 1986). The phosphoprotein is located within the virion nucleocapsid. The phosphoprotein of RS virus subgroup A is slightly larger than the phosphoprotein of RS virus subgroup B. This difference in molecular weight was demonstrated by immunoblot using this antibody (Gimenez et al., 1986). This antibody is useful for the diagnosis of RS virus infection since the phosphoprotein is abundant which makes the detection assays using this antibody highly sensitive.
Format: Liquid
Concentration: 1mg/ml
Storage buffer: Dulbecco’s media containing 20% Fetal Bovine serum (DH20) prepared as follows (for final volume of 300ml: 237ml DMEM plus 60 ml Fetal Bovine Serum plus 3ml L-Glutamine).
Storage conditions: -15° C to -25° C
Shipping conditions: Dry ice
References:

Gimenez et al. 1984. Journal General Virology, 65: 963-71. PMID: 6202832 Gimenez et al. 1986. Journal General Virology, 67: 863-70. PMID: 3517224 Gimenez et al. 1987. Journal General Virology, 68, 1267-75. PMID: 3572364

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