Cat. #152570
Anti-PLA2R [12-6-5]
Cat. #: 152570
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 3-4 weeks
Target: Phospholipase A2 receptor 1
Class: Monoclonal
Application: FACS ; IHC ; IF ; WB
Reactivity: Human
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Paul Brenchley
Institute: University of Manchester
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-PLA2R [12-6-5]
- Research fields: Cell signaling and signal transduction;Metabolism;Tissue-specific biology
- Clone: 1
- Tool sub type: Primary antibody
- Class: Monoclonal
- Purpose: Marker
- Conjugation: Unconjugated
- Reactivity: Human
- Host: Mouse
- Application: FACS ; IHC ; IF ; WB
- Description: Monoclonal antibody which detects PLA2R, the major antigen for idiopathic membranous nephropathy. Background and Research Application PLA2R is the major auto-antigen in the autoimmune kidney disease, membranous nephropathy. Detection of PLA2R antigen by IHC in the immune complexes present in the glomerular basement membrane is used as a diagnostic marker for the disease. PLA2R is also involved in clearance followed by suppression of their potent enzymatic activities of secretory phospholipas...
- Immunogen: Human PLA2R extracellular sequence N-C3 (containing domains N-terminal Cysteine rich-FibII-CTLD1-CTLD2-CTLD3). Genbank acc no. U17033; amino acids 20-663 Recombinant protein expressed in HEK 293-EBNA-1 cells
- Immunogen uniprot id: Q13018
- Isotype: IgG2b
- Myeloma used: Sp2/0-Ag14
Target Details
- Target: Phospholipase A2 receptor 1
- Tissue cell line specificity: Mouse
- Target background: Monoclonal antibody which detects PLA2R, the major antigen for idiopathic membranous nephropathy. Background and Research Application PLA2R is the major auto-antigen in the autoimmune kidney disease, membranous nephropathy. Detection of PLA2R antigen by IHC in the immune complexes present in the glomerular basement membrane is used as a diagnostic marker for the disease. PLA2R is also involved in clearance followed by suppression of their potent enzymatic activities of secretory phospholipas...
Applications
- Application: FACS ; IHC ; IF ; WB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: Store at -20° C frozen. Avoid repeated freeze / thaw cycles
- Shipping conditions: Shipping at 4° C
References
- Fresquet et al. 2017. Sci Rep. 7(1):6876. PMID: 28761153.
- PLA2R binds to the annexin A2-S100A10 complex in human podocytes.
- Obrisca et al. 2015. Biomed Res Int. 2015:249740. PMID: 26576418.
