Monoclonal antibody which detects the tumour suppressor Rb, when phosphorylated at residue Ser608.
| Inventor | Institute |
|---|---|
| Sibylle Mittnacht | The Institute of Cancer Research |
| Cat. #: | 153409 |
|---|---|
| Tool sub type: | Primary antibody |
| Unit size: | 100 ug |
| Cancer types: | Colorectal cancer, Breast cancer, Prostate cancer |
| Research Fields: | Apoptosis and autophagy;Cancer;Cell biology;Immunology |
| Application: | IHC ; IF ; WB |
| Target: | Rb (phospho Ser608) |
| Reactivity: | Human ; Mouse |
| Clone: | 51B7 |
| Host: | Mouse |
| Class: | Monoclonal |
| Alternate name: | Retinoblastoma 1; RB1; p15-Rb; RB; pp11; pRb; OSRC |
|---|---|
| Product description: | Monoclonal antibody which detects the tumour suppressor Rb, when phosphorylated at residue Ser608. |
| Conjugation: | Unconjugated |
| Isotype: | IgG1 |
| Molecular weight: | 111 kDa |
| Immunogen: | Synthetic peptide C-ADMYLSPLRSPK phosphorylated at S608, conugated to KLH |
| Immunogen Uniprot ID: | P06400 |
| Target background: | Retinoblastoma protein (Rb) is a tumour suppressor nuclear phosphoprotein capable of binding to DNA. It is phosphorylated on serine and threonine, but not on tyrosine residues. It forms a complex with SV40 large T antigen, adenovirus E1A, and human papilloma virus-16 E. One function of Rb is to prevent excessive cell growth by inhibiting cell cycle progression. Rb is also a recruiter of several chromatin remodelling enzymes such as methylases and acetylases. Rb protein may act by regulating transcription and loss of its function leads to uncontrolled cell growth. Aberrations in the RB gene have been implicated in cancers of breast, colon, prostate, kidney, nasopharynx, and leukaemia. Phosphorylation of Rb at S608 depends on CDK4. |
|---|
| Format: | Liquid |
|---|---|
| Concentration: | 1 mg/ml |
| Storage buffer: | PBS with 0.02% azide |
| Storage conditions: | Store at -20° C frozen. Avoid repeated freeze / thaw cycles |
| Shipping conditions: | Dry ice |
| References: |
Wadosky et al. 2016. J Mol Endocrinol. 56(3):273-90. PMID: 26862156. Silva et al. 2014. PLoS One. 9(6):e99897. PMID: 24936870. Repair of oxidative DNA damage, cell-cycle regulation and neuronal death may influence the clinical manifestation of Alzheimer's disease. Shilkaitis et al. 2013. Cancer Prev Res (Phila). 6(4):299-308. PMID: 23430755. Bettencourt-Dias et al. 2003. J Cell Sci. 116(Pt 19):4001-9. PMID: 12928330. Barrie et al. 2003. Anal Biochem. 320(1):66-74. PMID: 12895470. High-throughput screening for the identification of small-molecule inhibitors of retinoblastoma protein phosphorylation in cells. Heterogeneous proliferative potential in regenerative adult newt cardiomyocytes. |
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