#151456

Anti-PCNA [5E6/2]

Cat. #151456

Anti-PCNA [5E6/2]

Cat. #: 151456

Sub-type: Primary antibody

Unit size: 100 ug

Availability: 3-4 weeks

Target: Proliferating cell nuclear antigen, (PCNA), also known as cyclin or polymerase delta accessory protein.

Class: Monoclonal

Application: WB ; ELISA ; IP ; WB

Reactivity: Saccharomyces cerevisiae

Host: Mouse

£300.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Helle Ulrich

Institute: Cancer Research UK, London Research Institute: Clare Hall Laboratories

Tool Details
Target Details
Applications
Handling
References

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

  • Name: Anti-PCNA [5E6/2]
  • Cancer: Other
  • Cancers detailed: Broadly Applicable
  • Research fields: Cancer;Cell biology;Genetics
  • Clone: 5E6/2
  • Tool sub type: Primary antibody
  • Class: Monoclonal
  • Conjugation: Unconjugated
  • Molecular weight: 30 kDa
  • Strain: CD1
  • Reactivity: Saccharomyces cerevisiae
  • Host: Mouse
  • Application: WB ; ELISA ; IP ; WB
  • Description: Monoclonal antibody which binds PCNA, a tumour proliferation marker.
  • Immunogen: Purified recombinant His6-POL30 (PCNA) from budding yest, Saccharomyces cerevisiae, produced in Escherichia coli
  • Immunogen uniprot id: P12004
  • Isotype: IgG1
  • Myeloma used: Sp2/0-Ag14
  • Recommended controls: Wild type budding yeast expressing native POL30 (PCNA)

Target Details

  • Target: Proliferating cell nuclear antigen, (PCNA), also known as cyclin or polymerase delta accessory protein.
  • Molecular weight: 30 kDa
  • Tissue cell line specificity: Wild type budding yeast expressing native POL30 (PCNA)
  • Target background: Monoclonal antibody which binds PCNA, a tumour proliferation marker. Background and Research Application PCNA, also known as polymerase delta auxiliary protein, is essential for DNA replication and is involved in DNA excision and mismatch repair pathways. It is required for cellular DNA synthesis, and in vitro replication of SV40 DNA. It helps to co-ordinate the leading and lagging strand synthesis at the replication fork. PCNA binds to the CDK inhibitor p21, the structure-specific endonucle...

Applications

  • Application: WB ; ELISA ; IP ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Unit size: 100 ug
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: Store at -20° C frozen. Avoid repeated freeze / thaw cycles
  • Shipping conditions: Shipping at 4° C

References

  • Paul Solomon Devakumar et al. 2019. Nucleic Acids Res. 47(13):6826-6841. PMID: 31114918.
  • Schalbetter et al. 2015. Proc Natl Acad Sci U S A. 112(33):E4565-70. PMID: 26240319.
  • Kubota et al. 2011. Mol Cell Proteomics. 10(7):M110.005561. PMID: 21505101.
  • Quantitative proteomic analysis of chromatin reveals that Ctf18 acts in the DNA replication checkpoint.
  • Parker et al. 2008. EMBO J. 27(18):2422-31. PMID: 18701921.
  • SUMO modification of PCNA is controlled by DNA.
  • Davies et al. 2008. Mo...