Cat. #151293
Anti-p53 [Pab DO-7]
Cat. #: 151293
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 1-2 weeks
Target: p53 (N terminus)
Class: Monoclonal
Application: ELISA ; FACS ; IHC ; IF ; WB
Reactivity: Human
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: David Lane
Institute: University of Dundee
Primary Citation: Vojtesek et al. 1992. J Immunol Methods. 151(1-2):237-44. PMID: 1378473
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-p53 [Pab DO-7]
- Cancer: Other
- Cancers detailed: Broadly Applicable
- Research fields: Apoptosis and autophagy;Cancer;Cell biology;Genetics
- Clone: Pab DO-7
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Molecular weight: 53 kDa
- Reactivity: Human
- Host: Mouse
- Application: ELISA ; FACS ; IHC ; IF ; WB
- Description: A monoclonal p53 antibody, the epitope recognised by DO-7 is in the N terminus of p53. DO-7 can detect wild-type and mutant p53. DO-7 recognises three of the p53 isoforms (p53, p53beta, p53gamma).
- Immunogen: p53
- Immunogen uniprot id: P04637
- Isotype: IgG2b
- Recommended controls: MDA-MB-231 cell line
Target Details
- Target: p53 (N terminus)
- Molecular weight: 53 kDa
- Tissue cell line specificity: MDA-MB-231 cell line
- Target background: p53 is a crucial tumour suppressor involved in over 50% of cancers. It acts as a stress-responsive transcription factor and plays a vital role in regulating cell cycle arrest, promoting apoptosis, maintaining genomic stability, controlling the cell cycle, and inhibiting angiogenesis. Known as the "guardian of the genome," p53 prevents gene mutations. Mutations in the p53 gene are common in human cancers, resulting in dysfunctional proteins unable to bind to DNA. This loss of fun...
Applications
- Application: ELISA ; FACS ; IHC ; IF ; WB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: Store at -20° C frozen. Avoid repeated freeze / thaw cycles
- Shipping conditions: Shipping at 4° C
References
- Grass et al. 2019. Leuk Lymphoma. 60(4):886-893. PMID: 30457458.
- Elsensohn et al. 2018. Am J Surg Pathol. 42(3):372-375. PMID: 29112020
- Kanda et al. 2018. BMC Cancer. 18(1):657. PMID: 29907137.
- Murnyk et al. 2017. Oncotarget. 8(28):46348-46362. PMID: 28654422.
- Gavressea et al. 2017. Anticancer Res. 37(6):2947-2957. PMID: 28551632.
- Ilhan et al. 2016. J Vet Diagn Invest. PMID: 27016721.
- Alsafadi et al. 2016. Oncotarget. 7(11):12331-43.
