Cat. #153404
Anti-p53 [DO-13]
Cat. #: 153404
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 10-12 weeks
Target: Human TP53 / p53
Class: Monoclonal
Application: IHC ; IP ; WB
Reactivity: Human
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: David Lane
Institute: University of Dundee
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-p53 [DO-13]
- Alternate name: p53
- Cancer: Other
- Cancers detailed: Broadly Applicable
- Research fields: Apoptosis and autophagy;Cancer;Cell biology;Immunology
- Clone: DO-13
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Cell signalling pathway: p53
- Molecular weight: 53 kDa
- Reactivity: Human
- Host: Mouse
- Application: IHC ; IP ; WB
- Description: Mouse anti-human p53 antibody, clone DO-13 recognises the human cellular tumour antigen p53, also known as p53 tumour suppressor protein or NY-CO-13. p53 is a 393 amino acid ~53kDa cytoplasmic/ nuclear protein upregulated in response to DNA damage and is found in a wide variety of transformed cells. DO-13 binds to an epitope between amino acids 26-35.
- Immunogen: Recombinant human p53
- Isotype: IgG1
Target Details
- Target: Human TP53 / p53
- Molecular weight: 53 kDa
- Target background: p53 is a crucial tumour suppressor involved in over 50% of cancers. It acts as a stress-responsive transcription factor and plays a vital role in regulating cell cycle arrest, promoting apoptosis, maintaining genomic stability, controlling the cell cycle, and inhibiting angiogenesis. Known as the "guardian of the genome," p53 prevents gene mutations. Mutations in the p53 gene are common in human cancers, resulting in dysfunctional proteins unable to bind to DNA. This loss of fun...
Applications
- Application: IHC ; IP ; WB
Handling
- Format: Liquid
- Concentration: 0.9-1.1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15° C to -25° C
- Shipping conditions: Shipping at 4° C
References
- Hsieh et al. 2002. Mol Cell Biol. 22(1):78-93. PMID: 11739724.
- Vojtesek et al. 1995. Oncogene. 10(2):389-93. PMID: 7530828.