Cat. #151042
Anti-LewisX [29]
Cat. #: 151042
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 3-4 weeks
Target: Lewis X (CD15)
Class: Monoclonal
Application: FACS
Reactivity: Human
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Nancy Hogg
Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-LewisX [29]
- Alternate name: Fucosyltransferase 4; Galactoside 3-L-Fucosyltransferase; ELAM-1 Ligand Fucosyltransferase; FUC-TIV; FCT3A; ELFT; Stage-Specific Embryonic Antigen; Alpha (1,3) Fucosyltransferase; EC 2.4.1.65; Lewis X; SSEA-1; FUTIV; CD15; LeX
- Research fields: Cancer;Immunology
- Clone: 29
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Molecular weight: 83 kDa
- Strain: Balb/c
- Reactivity: Human
- Host: Mouse
- Application: FACS
- Description: Monoclonal antibody directed at against Lewis X antigen, with use in differentiating between leukaemia's and aids in the purification of stem cells.
- Immunogen: Fibronectin purified human monocytes .
- Immunogen uniprot id: P22083
- Isotype: IgM
- Myeloma used: Sp2/0-Ag14
Target Details
- Target: Lewis X (CD15)
- Molecular weight: 83 kDa
- Target background: Lewis X (CD15) is a branched pentasaccharide found on glycolipids, glycoproteins and proteoglycans of cells of gastric mucosa, nervous system, neutrophils, eosinophils, and monocytes. Lewis X is distributed abnormally in myeloid leukaemia's and is commonly used in the diagnosis of Hodgkin's disease. Lewis X is believed to enhance cell adhesion, either directly or by promoting integrin activation. Cells with high surface expression of Lewis X antigen, display strong self-aggregation, based on calcium-dependent Lewis X-Lewis X interaction. Anti-Lewis X (29) is useful for leukaemia typing and the purification of stem cells.
Applications
- Application: FACS
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: Store at -20° C frozen. Avoid repeated freeze / thaw cycles
- Shipping conditions: Shipping at 4° C
References
- Bernstein I & Self S. Report of the myeloid section of the Second International Workshop on Leucocyte Typing II, Human leucocyte Differentiation Antigens. Published by Springer-Verlag, New York. 1986.
