Anti-Involucrin [SY5] mAb

Clone SY5 recognizes a protein of 66kDa-170kDa, identified as involucrin.

Contributors

Inventor Institute
Fiona Watt Cancer Research UK, Lincoln's Inn Fields Institute
Cat. #: 151163
Tool sub type: Primary antibody
Unit size: 100 ug
Cancer types: Skin cancer
Research Fields: Cancer;Cell signaling and signal transduction
Application: IF ; FACS ; IHC ; IF ; IP ; WB
Target: Involucrin
Reactivity: Dog ; Human ; Pig ; Primate
Clone: SY5
Host: Mouse
Class: Monoclonal
Primary citation: Hudson et al. 1992. Hybridoma. 11(3):367-379. PMID: 1500072.
Alternate name: Anti-Involucrin; involucrin antibody
Product description: Clone SY5 recognises a protein of 66kDa-170kDa, identified as involucrin. Involucrin is a protein precursor of the epidermal cornified envelope and a differentiation marker of human keratinocytes. Involucrin expression is altered in pathological conditions such as psoriasis and other benign epidermal hyperplasias. This mAb stains the involucrin in a variety of sizes: 170kDa in MCF-7 cells, 66kDa in dog, and a doublet of 105kDa in pig. It's epitope maps between codon 421-568 of human involucrin. SY5 is useful for studies of changes in involucrin expression in pathological studies.
Conjugation: Unconjugated
Isotype: IgG1 kappa
Molecular weight: 66-170 kDa
Immunogen: Purified involucrin from human keratinocytes.
Myeloma used: P3X63Ag8
Target background: In Western blotting of cultured human keratinocytes, this MAb reacts with a 120kDa protein. It stains the involucrin in a variety of sizes: 170kDa in MCF-7 cells, a doublet of ~115kDa and 150kDa in gorilla and owl monkey, 66kDa in dog, and a doublet of 105kDa in pig. Its epitope maps between codon 421-568 of human involucrin. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures, all cells that have left the basal layer express it.Involucrin is a protein precursor of the epidermal cornified envelope and a differentiation marker of human keratinocytes. SY5 is useful for studies of changes in involucrin expression in pathological studies. Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias, involucrin expression begins closer to the basal layer than normal; expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix.
Format: Liquid
Concentration: 1 mg/ml
Storage buffer: PBS with 0.02% azide
Storage conditions: -15° C to -25° C
Shipping conditions: Dry ice
References:

Zhong et al. 2016. Cell. 167(1):187-202. PMID: 27662089.

Morales et al. 2016. Toxicol In Vitro. 36(8):89-96. PMID: 27448499.

Raj et al. 2016. Int J Cosmet Sci. 39(1):2-10. PMID: 27079667.

Golinski et al. 2015. Cells Tissues Organs. 200(3-4):227-239. PMID: 26303436.

Ravindran et al. 2015. Head Neck. 37(7):982-993. PMID: 24700702.

Creppe et al. 2012. Mol Cell Biol. 32(8):1442-1452. PMID: 22331466.

White et al. 2010. J Immunol. 185(8):4769-4776. PMID: 20861360.

Skoog et al. 2009. J Invest Dermatol. 129(1):119-130. PMID: 18633436.

Hudson et al. 1992. Hybridoma. 11(3):367-379. PMID: 1500072.

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