Cat. #151180
Anti-CyclinB1 [V92.1]
Cat. #: 151180
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 1-2 weeks
Target: Cyclin B1
Class: Monoclonal
Application: FACS ; IHC ; IF ; IP ; WB
Reactivity: Hamster ; Human ; Mouse
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Julian Gannon
Institute: Cancer Research UK, London Research Institute: Clare Hall Laboratories
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-CyclinB1 [V92.1]
- Alternate name: CCNB, CCNB1, CCNB1_HUMAN, G2 Mitotic Specific Cyclin B1Human Entrez Gene ID 891 Human SwissProt P14635 Human Unigene 2396 Human Gene Symbol CCNB1Human Chromosome Location 5q13.2
- Research fields: Cancer;Cell biology;Genetics
- Clone: V92.1
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Molecular weight: 55-62 kDa
- Reactivity: Hamster ; Human ; Mouse
- Host: Mouse
- Application: FACS ; IHC ; IF ; IP ; WB
- Description: Clone V92.1, recognizes a protein of 55-62kDa, identified as cyclin B1.
- Immunogen: Hamster Cyclin B1
- Isotype: IgG1 kappa
- Myeloma used: Sp2/0-Ag14
- Recommended controls: Cell line in logarithmic growth phase. Tonsil or testicular, endometrial, prostate or ovarian carcinoma.
Target Details
- Target: Cyclin B1
- Molecular weight: 55-62 kDa
- Tissue cell line specificity: Cell line in logarithmic growth phase. Tonsil or testicular, endometrial, prostate or ovarian carcinoma.
- Target background: Cyclins bind to and regulate the activity of the Cyclin Dependent Protein Kinases (CDKs). Cyclin B1 is a marker of cell proliferation and a key component of the cell cycle progression machinery. Cyclin B1 may prove to be a prognostic marker in neoplasia. V92.1 may be used to immunoprecipitate active CDK1/cyclin complexes.
Applications
- Application: FACS ; IHC ; IF ; IP ; WB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15° C to -25° C
- Shipping conditions: Shipping at 4° C
References
- Garner et al. 2002. Oncogene. 21(53):8089-104. PMID: 12444545.
- Delta MEKK3:ER* activation induces a p38 alpha/beta 2-dependent cell cycle arrest at the G2 checkpoint.
- Minemoto et al. 2001. Exp Cell Res. 262(1):37-48. PMID: 11120603.
- Characterization of adriamycin-induced G2 arrest and its abrogation by caffeine in FL-amnion cells with or without p53.
