AF 3.1 is recommended for the immunoprecipitation of active APC.
| Inventor | Institute |
|---|---|
| Julian Gannon | Cancer Research UK, London Research Institute: Clare Hall Laboratories |
| Cat. #: | 151232 |
|---|---|
| Tool sub type: | Primary antibody |
| Unit size: | 100 ug |
| Research Fields: | Biochemistry;Cell biology |
| Application: | ELISA ; FACS ; IHC ; IP ; WB |
| Target: | Cell division cycle protein 27 (cdc27) |
| Reactivity: | Mammalian ; Xenopus laevis |
| Clone: | AF 3.1 |
| Host: | Mouse |
| Class: | Monoclonal |
| Alternate name: | Cell Division Cycle 27; Anaphase Promoting Complex Subunit; DS143E; D17S978E; CDC27Hs; ANAPC3; H-NUC; APC3; Anaphase-Promoting Complex; Cell Division Cycle 27 Homolog; CDC27 Homolog; Nuc2 Homolog; HNUC; NUC2 |
|---|---|
| Product description: | AF 3.1 is recommended for the immunoprecipitation of active APC. |
| Conjugation: | Unconjugated |
| Isotype: | IgG2b |
| Molecular weight: | 80 kDa |
| Immunogen: | A peptide corresponding to the C-terminal 10 residues of human cdc27 coupled to KLH. |
| Myeloma used: | Sp2/0-Ag14 |
| Target background: | Cdc27 is a component of the anaphase promoting complex (APC) which is responsible for destroying proteins, including cyclins, which are involved in mitosis. |
|---|
| Format: | Liquid |
|---|---|
| Concentration: | 1 mg/ml |
| Storage buffer: | PBS with 0.02% azide |
| Storage conditions: | -15° C to -25° C |
| Shipping conditions: | Dry ice |
| References: |
Mateo F et al. Oncogene. 2009 Jul 23 28(29):2654-66. Ahlskog et al. 2010. Mol Cell Biol. 30(24):5608-20. PMID: 20937767. Anaphase-promoting complex/cyclosome participates in the acute response to protein-damaging stress. Holt et al. 2010. Development. 137(8):1297-304. PMID: 20223764. Spatial regulation of APCCdh1-induced cyclin B1 degradation maintains G2 arrest in mouse oocytes. Hochegger et al. 2007. J Cell Biol. 178(2):257-68. PMID: 17635936. An essential role for Cdk1 in S phase control is revealed via chemical genetics in vertebrate cells. Yamano et al. 1998. EMBO J. 17(19):5670-8. PMID: 9755167. The role of the destruction box and its neighbouring lysine residues in cyclin B for anaphase ubiquitin-dependent proteolysis in fission yeast: defining the D-box receptor. |
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