Cat. #151302
Anti-Cdc20 [BA8]
Cat. #: 151302
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 3-4 weeks
Target: Cell division cycle protein 20 (cdc20)
Class: Monoclonal
Application: ELISA ; FACS ; WB
Reactivity: Xenopus laevis
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Julian Gannon
Institute: Cancer Research UK, London Research Institute: Clare Hall Laboratories
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-Cdc20 [BA8]
- Alternate name: APC/C activator protein CDC2; Cell division control protein 2; YGL116W; Cell Division Cycle 2; P55CDC; CDC2 Cell Division Cycle 2; BA276H19.3; CDC2A
- Research fields: Biochemistry;Cell biology
- Clone: BA8
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Strain: Balb/c
- Reactivity: Xenopus laevis
- Host: Mouse
- Application: ELISA ; FACS ; WB
- Description: Fizzy protein (cdc20) is associated with the activation of the anaphase-promoting complex (APC) at the onset of anaphase.
- Immunogen: Peptide from the carboxy terminus of the protein.
- Isotype: IgG2a
- Myeloma used: Sp2/0-Ag14
Target Details
- Target: Cell division cycle protein 20 (cdc20)
- Target background: Fizzy protein (cdc20) is associated with the activation of the anaphase-promoting complex (APC) at the onset of anaphase.
Applications
- Application: ELISA ; FACS ; WB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15° C to -25° C
- Shipping conditions: Shipping at 4° C
References
- Ohe et al. 2010. Mol Biol Cell. 21(6):905-13. PMID: 20089832.
- Emi2 inhibition of the anaphase-promoting complex/cyclosome absolutely requires Emi2 binding via the C-terminal RL tail.
- Sironi et al. 2001. EMBO J. 20(22):6371-82. PMID: 11707408.
- Mad2 binding to Mad1 and Cdc20, rather than oligomerization, is required for the spindle checkpoint.
- Lorca et al. 1998. EMBO J. 17(13):3565-75. PMID: 9649427.
- Fizzy is required for activation of the APC/cyclosome in Xenopus egg extracts.
