![Immunofluorescence on endothelial cells transfected with mNrp1-mRFP and Rab5-GFP and then incubated with anti-CD49e [SNAKA51]. mNrp1-mRFP and active a5b1 colocalize into Rab5-GFP-positive early endosomes as shown in merging (arrowheads). Lower panels are magnifications of the boxed areas shown in the upper panels. Taken from Valdembri et al. 2009. PLoS Biol. 7(1)](https://cancertools.org/wp-content/uploads/bd8af2d6-78ba-4733-a68f-7e14cbefc274.png)
Cat. #152487
Anti-CD49e [SNAKA51]
Cat. #: 152487
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 3-4 weeks
Target: Integrin alpha5 (CD49e)
Class: Monoclonal
Application: IF ; Fn ; WB
Reactivity: Human
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Martin Humphries
Institute: University of Manchester
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-CD49e [SNAKA51]
- Alternate name: Integrin Subunit Alpha 5; Fibronectin Receptor Subunit Alpha; CD49 Antigen-Like Family Member E; Integrin Alpha-F; VLA-5; FNRA; Very Late Activation Protein 5; Alpha Subunit; Fibronectin Receptor; Alpha Polypeptide; Fibronectin Receptor; Alpha Subunit; Fibronectin Receptor; Alpha Polypeptide; Integrin Alpha; CD49e Antigen; CD49e; VLA5A
- Research fields: Apoptosis and autophagy;Cell biology;Cell signaling and signal transduction;Immunology
- Clone: SNAKA51
- Tool sub type: Primary antibody
- Class: Monoclonal
- Purpose: Marker
- Conjugation: Unconjugated
- Reactivity: Human
- Host: Mouse
- Application: IF ; Fn ; WB
- Description: CD49e is the integrin alpha 5 chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. Alpha chain 5 undergoes post translational cleavage in the extracellular domain to yield disulfide linked light and heavy chains that join with beta 1 to form a fibronectin receptor that is known variously: in lymphocytes as very late (activation) antigen 5 (VLA5); in platelets as glycoprotein IcIIa; and in fibroblasts as extracellular matrix receptor 6 (ECM...
- Immunogen: Whole cells corresponding to human Integrin alpha5.
- Isotype: IgG2a kappa
- Myeloma used: P3X63Ag8.653
- Recommended controls: HeLa cells
Target Details
- Target: Integrin alpha5 (CD49e)
- Tissue cell line specificity: Mouse
- Target background: CD49e is the integrin alpha 5 chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. Alpha chain 5 undergoes post translational cleavage in the extracellular domain to yield disulfide linked light and heavy chains that join with beta 1 to form a fibronectin receptor that is known variously: in lymphocytes as very late (activation) antigen 5 (VLA5); in platelets as glycoprotein IcIIa; and in fibroblasts as extracellular matrix receptor 6 (ECM...
Applications
- Application: IF ; Fn ; WB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15° C to -25° C
- Shipping conditions: Shipping at 4° C
References
- Valdembri et al. 2009. PLoS Biol. 7(1):e25. PMID: 19175293.
- Neuropilin-1/GIPC1 signaling regulates alpha5beta1 integrin traffic and function in endothelial cells.
- Clark et al. 2005. J Cell Sci. 118(Pt 2):291-300. PMID: 15615773.
- A specific alpha5beta1-integrin conformation promotes directional integrin translocation and fibronectin matrix formation.
