#153171

Anti-C-NAP1/CEP250 [6F2C8]

Cat. #153171

Anti-C-NAP1/CEP250 [6F2C8]

Cat. #: 153171

Unit size: 100 ug

Target: Centrosomal Nek2-Associated Protein 1

Class: Monoclonal

Application: IHC ; WB ; IHC ; IP ; WB

Reactivity: Human

Host: Mouse

£300.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Ciaran Morrison

Institute: National University of Ireland, Galway ; Centre For Chromosome Biology

Tool Details
Target Details
Applications
Handling
References

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

  • Name: Anti-C-NAP1/CEP250 [6F2C8]
  • Alternate name: Centrosome-associated protein CEP25; Centrosomal Protein 25; Centrosomal Protein 25kDa; Centrosomal Nek2-Associated Protein 1; 25 KDa Centrosomal Protein; Centrosomal Protein 2; C-NAP1; CNAP1
  • Research fields: Cell biology;Immunology
  • Clone: 6F2C8
  • Class: Monoclonal
  • Conjugation: Unconjugated
  • Molecular weight: 250
  • Reactivity: Human
  • Host: Mouse
  • Application: IHC ; WB ; IHC ; IP ; WB
  • Description: Centrosomal Protein 250 (CEP250), also known as Centrosomal Nek2-Associated Protein 1 (C-NAP1), is a core centrosomal protein required for centriole-centriole cohesion during interphase of the cell cycle. CEP250 dissociates from the centrosomes when parental centrioles separate at the beginning of mitosis. The protein associates with and is phosphorylated by NIMA-related kinase 2, which is also associated with the centrosome.
  • Immunogen: Recombinant human C-NAP1 amino acids 1513-1750 (bacterially expressed from pGEX4T as a GST fusion, then thrombin-cleaved and purified).
  • Isotype: IgG1 kappa
  • Myeloma used: Sp2/0-Ag14
  • Recommended controls: Immunofluorescence and Western Blot: Jurkat cells, hTERT-RPE1 cells, Immunofluorescence only: PC3 cells, DU145 cells, PWR1E cells, Peripheral Blood Mononuclear Cells (PBMCs)

Target Details

  • Target: Centrosomal Nek2-Associated Protein 1
  • Molecular weight: 250
  • Target background: Centrosomal Protein 250 (CEP250), also known as Centrosomal Nek2-Associated Protein 1 (C-NAP1), is a core centrosomal protein required for centriole-centriole cohesion during interphase of the cell cycle. CEP250 dissociates from the centrosomes when parental centrioles separate at the beginning of mitosis. The protein associates with and is phosphorylated by NIMA-related kinase 2, which is also associated with the centrosome.

Applications

  • Application: IHC ; WB ; IHC ; IP ; WB

Handling

  • Format: Liquid
  • Concentration: 0.9-1.1mg/ml
  • Unit size: 100 ug
  • Storage buffer: DMEM + 10% FBS + hypoxanthine supplement + non-essential amino acids
  • Storage conditions: -20° C
  • Shipping conditions: Shipping at 4° C

References

  • Flanagan et al. 2017. Mol Biol Cell. :. PMID: 28100636