![Staining of zebrafish skin using Anti-AKT1 [V7]. AKT1 can be seen to localise in the cytoplasm.](https://cancertools.org/wp-content/uploads/9f061882-d7de-47a3-8b27-2b1357f5af39.jpg "9f061882-d7de-47a3-8b27-2b1357f5af39.jpg")
Cat. #152617
Anti-AKT1 [V7]
Cat. #: 152617
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 1-2 weeks
Target: V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1)
Class: Monoclonal
Application: ELISA ; IHC ; WB
Reactivity: Zebrafish
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Ayham Alnabulsi
Institute: Vertebrate Antibodies Limited
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-AKT1 [V7]
- Research fields: Apoptosis and autophagy;Cancer;Cell biology;Cell signaling and signal transduction;Genetics;Metabolism;Neurobiology
- Clone: V7
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Strain: Balb/c
- Reactivity: Zebrafish
- Host: Mouse
- Application: ELISA ; IHC ; WB
- Description: The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival.
- Immunogen: Ovalbumin-conjugated synthetic peptide KDPMQRLGGG (C-terminal sequence)
- Isotype: IgG2a kappa
- Myeloma used: P3X63Ag8.653
- Recommended controls: ELISA - peptide immunogen; WB - whole organism lysate; IHC - zebrafish embryo
Target Details
- Target: V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1)
- Tissue cell line specificity: ELISA - peptide immunogen; WB - whole organism lysate; IHC - zebrafish embryo
- Target background: The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival.
Applications
- Application: ELISA ; IHC ; WB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15° C to -25° C
- Shipping conditions: Shipping at 4° C
