Anti-AKT1 [V7]

The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1.

Contributors

Inventor Institute
Ayham Alnabulsi Vertebrate Antibodies Limited
SKU: 152617
Product description: The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival.
Conjugation: Unconjugated
Isotype: IgG2a kappa
Immunogen: Ovalbumin-conjugated synthetic peptide KDPMQRLGGG (C-terminal sequence)
Myeloma used: P3X63Ag8.653
Cat. #: 152617
Tool sub type: Primary antibody
Unit size: 100 ug
Research Fields: Apoptosis and autophagy; Cancer; Cell biology; Cell signaling and signal transduction; Genetics; Metabolism; Neurobiology
Application: ELISA ; IHC ; WB
Target: V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1)
Reactivity: Zebrafish
Clone: V7
Host: Mouse
Class: Monoclonal
Target background: The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival.
Format: Liquid
Concentration: 1 mg/ml
Storage buffer: PBS with 0.02% azide
Storage conditions: -15° C to -25° C
Shipping conditions: Dry ice

Images

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Staining of zebrafish skin using Anti-AKT1 [V7]. AKT1 can be seen to localise in the cytoplasm.

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