Melan-Ink4a-Arf4 cell line

melan-Ink4a-Arf4 is an immortal melanocyte cell line from the skin of neonatal Ink4a-Arf–/– C57BL/6J mice

Contributors

Inventor Institute
Elena Sviderskaya St. George's University London
Cat. #: 161995
Organism: Mouse
Tissue: Skin
Model: Immortalised non-cancerous cell lines
Morphology: Small, oval-fusiform
Primary citation: Sviderskaya EV, et al. J Natl Cancer Inst. 2002 Mar 20;94(6):446-54. PMID: 11904317
Product description: The melan-Ink4a-Arf1, -2, -3 and -4 are immortalised mouse melanocyte lines derived from the skin of neonatal Ink4a-Arf–/– C57BL/6J mice. Each line was derived independently from a different littermate of the same background. These cells are visibly pigmented and display morphology typical of normal melanocytes. The INK4A-ARF locus encodes two inhibitors of cellular growth (p16INK4A and ARF) and is often mutated in a range of human cancers including melanoma. Therefore, these melanocytes can be used as a model of normal skin melanocytes in studies focussing on skin cancers and melanomas. They are also a useful tool for studies of pigmentation genetics and as a control for numerous other lines in the collection, as many of these have also been derived from mice that are null for Ink4a-Arf.
Storage conditions: liquid N2
Growth medium: RPMI + FBS (10%) + TPA (200 nM) + cholera toxin (CT) (200 pM)
References:

Sviderskaya EV, et al. J Natl Cancer Inst. 2002 Mar 20

94(6):446-54. PMID: 11904317

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