Cat. #161939
SK-UT-1B Cell Line
Cat. #: 161939
Availability: 8-10 weeks
Tissue: Uterus; Endometrium
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Lloyd J. Old, Germain Trempe
Institute: Memorial Sloan-Kettering Cancer Center (MSK)
Primary Citation: Fogh et al. 1977. Journal of the National Cancer Institute. 59.1.221. PMID: 327080.
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: SK-UT-1B Cell Line
- Cancer: Sarcoma
- Parental cell: This is a subline of the SK-UT-1 cell line. The parental cell line was established in 1972 from a 75-year-old Caucasian female with a uterine mixed mesodermal tumor consistent with leiomyosarcoma grade III.
- Gender: Female
- Tissue: Uterus; Endometrium
- Donor: This cell line was established in 1972 from a 75-year-old Caucasian female with a uterine mixed mesodermal tumor consistent with leiomyosarcoma grade III.
- Morphology: Epithelial
- Growth properties: Adherent
- Products or characteristics of interest: Karyotype: (P27) hypodiploid to diploid. Isoenzymes: AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1-2 Me-2, 1-2 PGM1, 1 PGM3, 1
- Description: SK-UT-1B is a subline of the SK-UT-1 human uterine leiomyosarcoma cell line and grows in adherent culture.
- Application: 3D cell culture
- Biosafety level: 1
Target Details
- Target: Antigen expression: Blood type B; Rh+
Applications
- Application: 3D cell culture
Handling
- Growth medium: 1:1 mixture of ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003, and F12 Medium then supplemented with fetal bovine serum to a final concentration of 10%.
- Temperature: 37° C
- Atmosphere: 5% CO2 in air
- Shipping conditions: Dry Ice
- Storage medium: Complete growth medium supplemented with 5% DMSO
- Str profiling: Amelogenin: XCSF1PO: 10,11D13S317: 10,13D16S539: 12,14D5S818: 10,11D7S820: 9,10TH01: 7TPOX: 8vWA: 16D3S1358: 15,16D21S11: 29,32.2D18S51: 11,17Penta_E: 17Penta_D: 11,14D8S1179: 14,15FGA: 22,23D19S433: 12,13D2S1338: 19,21
References
- Mao et al. 2008. Cancer letters. 267(1):49-54. PMID: 18407410.
- Chen et al. Cancer genetics and cytogenetics. 33(1):77-81. PMID: 3383166.
