MM165 cell line

Derived from a lymph node metastasis of superficial spreading melanoma (SSM). Low sensitivity to MEKi

Contributors

Inventor Institute
Ghanem Elias, Ahmad Najem Universit?Š libre de Bruxelles
Cat. #: 161627
Tool sub type: 10XUAS IVS p10
Cancer types: Skin cancer;Skin cancer
Research Fields: Cancer;Cancer
Organism: Human
Tissue: Lymphatic
Gender: Female
Model: NRAS Q61K
Growth properties: Adherent
Cancer Types In Detail: Melanoma;Melanoma
Primary citation: Leucci et al. 2016. Nature 531, 518–522. PMID: 27008969
Product description: Cell Pigmentation: Unpigmented cell line. Drug Response: Low sensititvty to MEKi
Gender: Female
CRISPR: No
Conditional: Yes
Parental cell line: Ham's F10 melanoma culture medium (HMCM)
Model description: This cell line displays an invasive/Mesenchymal_like phenotype
disease: Cancer;Cancer
Initial handling information: The frozen culture should be stored in liquid nitrogen upon arrival or thaw the vial and initiate the culture as soon as possible upon receipt
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry Ice
Growth medium: Ham’s F10 supplemented with 10% of fetal calf serum (FCS), with L-glutamine (1mM), penicillin (100 IU/ml), and streptomycin (100 Â?g/ml). Renewal 2 to 3 times per week
Subculture routine: Split sub-confluent cultures (70-80%) 1:2 to 1:5
Temperature: 37° C
Atmosphere: 5% CO2
Mycoplasma free: Yes
Biosafety level: 2
References:

Sabbah et al. 2021. Mol Cancer Res. 19(7):1221-1233. PMID: 33741716

Krayem et al. 2014. Eur J Cancer. 50(7):1310-20. PMID: 24559688

Krayem et al. 2018. Oncotarget. 9(61):31888-31903. PMID: 30159130

Soumoy et al. 2020. Cancers (Basel). 12(5):1323. PMID: 32455924

Porcelli et al. 2015. J Transl Med. 13:26. PMID: 25623468

Wouterset al. 2020. Nat Cell Biol. 22(8):986-998. PMID: 32753671

Verfaillie et al. 2015. Nat Commun 6, 6683. PMID: 25865119

Aftimos et al. 2013. Int J Oncol. 43(3)…

Citations And Reviews

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