MCF10A-AKTm-Control cell line

Non-tumorigenic immortalized breast epithelial cell stably overexpressing mutant cancer-related gene, AKT1 (AKT serine/threonine kinase 1)

Contributors

Inventor Institute
Medical-Industrial Translational Research Center Fukushima Medical University
SKU: 161209
Product description: Non-tumorigenic immortalized breast epithelial cell stably overexpressing mutant cancer-related gene, AKT1 (AKT serine/threonine kinase 1). AKT1, also referred to as protein kinase B, is a known oncogene. AKT activation relies on the PI3K pathway, and is recognized as a critical node in the pathway. Mutations in this gene are associated with multiple types of cancer and excessive tissue growth including Proteus syndrome and Cowden syndrome 6, and breast, colorectal, and ovarian cancers.The E17 hotspot is the most characterized of AKT1 mutations, and has been shown to result in activation of the protein. Mutations in AKT1 have also been shown to confer resistance to allosteric kinase inhibitors in vitro.
Alternate name: 06M09001
Cellosaurus ID: CVCL_C7Q1
Parental cell line: MCF10A, a non-tumorigenic cell line from human mammarygland epithelium
Model description: Control
Cat. #: 161209
Tool sub type: Continuous
Research Fields: Cancer; Cell biology
Organism: Human
Tissue: Mammarygland epithelium
Morphology: Epithelial
Growth properties: Adherent
Cancer Types In Detail: Non-tumorigenic
Initial handling information: Thaw the vial at 37 °C in a water bath?then seed cells into a T75 flask. Culture in a humidified Incubator at 37 ? with 5% CO2 in air. Culture medium should be changed every 2 to 3 days.
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Growth medium: DMEM/Ham’s F-12 supplemented with 5% heat-inactivated horse serum, 10 Â?g/ml insulin (human, recombinant), 5 Â?M forskolin, 0.5 Â?g/ml hydrocortisone, 20 ng/ml EGF (human, recombinant), 100 U/ml penicillin, and 100 Â?g/ml streptomycin
Temperature: 37° C
Atmosphere: Humidified incubator with 5% CO2
Storage medium: CELLBANKER 2 (Zenogen pharma)
Mycoplasma free: Yes
Biosafety level: 1

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