UOL-Hep-Rho0 cell line

This cell line consists of HepG2, Rho 0 line. This line has potential for researchers to use as a fusion partner with their choice of mitochondrial donor

Contributors

Inventor Institute
Amy Chadwick University of Liverpool, Molecular and Clinical Pharmacology
Cat. #: 161136
Research Fields: Drug development
Organism: Human
Tissue: Liver
Growth properties: Adherent
Product description: This cell line consists of HepG2 cybrid cell lines negative for cellular mitochondrial DNA, fused with different platelet partners (platelets contain only mitochondrial DNA and no nuclear DNA). Each cybrid cell line has a combination of SNPs in mitochondrial DNA, relevant to a variety of diseases. Cybrid cell line panel has potential use for drug testing
Additional notes: Parental cell line was purchased from ECACC
Parental cell line: HepG2
Model description: HepG2 Rho0 cell line (devoid of mitochondrial DNA)
Growth medium: DMEM/F-12 + GlutaMAXTM supplemented with FBS (10 % v/v), L-glutamine (4?mM), sodium pyruvate (1?mM), HEPES (2?mM) and uridine (500 ?M)
Subculture routine: Cells are subcultured as required (1:6), via trypsinisation, but media is changed twice weekly

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