Recombinant CHO-K1 cell line with integrated Technology for enhanced production of second generation Human Erythropoietin protein called Darbepoetin (Darbe), in Adherent Cells

Contributors

Inventor Institute
Saiyed Taslimarif ; Patel Paresh Brijalal ; Mukhopadhyaya Rabindranath The Centre for Cellular and Molecular Platforms C-CAMP
Cat. #: 160886
Research Fields: Drug development
Organism: Hamster
Tissue: Ovary
Alternate name: haematopoietin, or haemopoietin
Conditional: No
Production details: The CHO-K1 cells were transduced with the two different lentiviral vectors for stable constitutive expression of 1) Darbe gene and 2) a ShRNA targeting a specific protease that can cleave the Darbe protein. G418 and Puromycin were used for selection of stable clones. The Darbe is expressed without any affinity tag and is actively secreted in the culture supernatant.
Parental cell line: CHO-K1
Disease: Therapeutics
Growth medium: F12 media supplemented with 10% FBS and 1% of 5000U/ml Penstrep (penicillin G and streptomycin) antibiotic. The cells are grown in a CO2 incubator at 37?‚°C with 5% CO2.
Temperature: 37° C
Atmosphere: 5% CO2
References:

A VECTOR, A HOST CELL AND A METHOD THEREOF (PCT/IB2019/052213)

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