Cat. #160738
iPSC healthy control for neuronal differentiation (F002.1A.13) cell line
Cat. #: 160738
Unit size: 1x10^6 cells / vial
Availability: 10-12 weeks
Organism: Human
Tissue: Fibroblast
Disease: Cerebellar diseases
Model: Stem Cells
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Maria Carmo-Fonseca
Institute: Instituto de Medicina Molecular Jo?Âo Lobo Antunes
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: iPSC healthy control for neuronal differentiation (F002.1A.13) cell line
- Research fields: Genetics;Neurobiology;Stem cell biology
- Parental cell: Human fibroblasts
- Organism: Human
- Tissue: Fibroblast
- Disease: Cerebellar diseases
- Growth properties: Healthy control
- Model: Stem Cells
- Conditional: No
- Description: Healthy control ÄË?Â? optimized for cerebellar neurons differentiation. Generation of electrophysiologically active GABAergic and glutamatergic cerebellar neurons in long-term cultures without co-culturing with other cell types. Relevant model for drug screening and for study specific pathways involved with the development of cerebellar diseases such as ataxias, a group of disorders that affect many children and adults worldwide.
- Production details: Reprogramming of human induced pluripotent stem cells from a skin biopsy of a female healthy donor.
Target Details
- Target: Healthy control
Handling
- Format: Frozen
- Growth medium: Cells are cultured on MatrigelTM (Corning)-coated plates with mTeSRTM1 Medium (StemCell Technologies). Medium is changed daily. Cells are passaged every 3ÄË?Â?Â4 days (when the colonies covered approximately 85% of the surface area of the culture dish) using 0.5 mM EDTA dissociation buffer (Life Technologies). Cells are incubated at 37 ÄË?ÂÄÂC in a humidified 5% CO2 incubator.
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
