Bovine Type II Alveolar Epithelial (B2AE) cell line

Contributors

Inventor Institute
Diane Lee University of Surrey
SKU: 157737
Product description: Differentiates into Alveolar Type I (ATI)-like cells when cultured at air-liquid interface, generating a mixed population of Alveolar Type II (ATII) and ATI in a pseudostratified epithelial cell layer. Used in co-culture with bovine pulmonary arterial endothelial cells as a model of the bovine alveolus.
Production details: Generated by the lentiviral transduction of Bmi1 and hTERT into primary bovine alveolar type II epithelial cells at passage 1. Proliferation rate as for primary.
Cat. #: 157737
Unit size: 1×10^6 cells / vial
Research Fields: Immunology
Tissue: Alveolus
Model: Immortalised Line
Growth properties: Cobblestone. Fixed (2D), 3D (matrigel) or air-liquid interface (ALI)
Format: Frozen
Shipping conditions: Dry ice
Growth medium: Small airway growth medium (Lonza) or Small airway epithelial cell medium (Promocell). 95 % air/5 % CO2, 37oC humidified incubator. Subculture routine: 0.25 % trypsin/1 mM EDTA 5 minutes at 37oC. Neutralise with medium/10 % FBS. Spin, count and subculture ÂĚ?Ÿ dilution to minimum of 3 x 10,000/cm2. Yield at subculture: ~5 x 10,000/cm2.

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