pFLAG-HBx vector

The addition of the FLAG-epitope tag allows for detection in western and immunofluorescent cell-based studies, providing better detection ability than current anti-HBx antibodies.

Contributors

Inventor Institute
Michael Bouchard Drexel University
SKU: 156514
Product description: This vector can be used to express hepatitis B virus HBx protein in a variety of primary and cancer cells. The addition of the FLAG-epitope tag allows for detection in western and immunofluorescent cell-based studies and provides better detection ability than can currently be achieved with commercially available anti-HBx antibodies.
Alternate name: nonstructural protein X, protein X, peptide X, pX
Cat. #: 156514
Tool sub type: pcDNA3.1
Research Fields: Cancer; Microbiology
Application: p
Target: HBx with FLAG tag at N-terminus
Backbone size without insert: 5428
Bacterial resistance: Ampicillin
Selectable markers: Neomycin
Application: p
Application notes: Multi-functional hepatitis B viral protein, non-structural protein X, that modulates mitochondrial function in host hepatocytes interfering with host anti-viral defenses, promoting viral transcription, and contributing to hepatocellular carcinoma development.
Shipping conditions: Dry Ice
References:

Clippinger et al. 2008. J Virol. 82(14):6798-811. PMID: 18448529.

McClain et al. 2007. J Virol. 81(21):12061-5. PMID: 17699583.

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