HEK293T BRE-Luc/TK Renilla cell line

The cell line stably expresses BMP responsive element (BRE)-Luciferase. The cell line also contains a Renilla reporter driven by the thymidine kinase (TK) promoter to act as an internal control.

Contributors

Inventor Institute
Caroline Hill The Francis Crick Institute
Cat. #: 156440
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Research Fields: Cancer;Cell signaling and signal transduction;Developmental biology
Organism: Human
Tissue: Kidney
Model: Reporter
Product description: The human embryo kidney cell line HEK293T modified for use as a reporter cell line for bone morphogenetic protein (BMP) signalling. The cell line stably expresses BMP responsive element (BRE)-Luciferase. The cell line also contains a Renilla reporter driven by the thymidine kinase (TK) promoter to act as an internal control. BMP is a transforming growth factor beta (TGF-beta) superfamily member.
Conditional description: Luciferase is expressed in the presence of bone morphogenetic protein (BMP) signalling
Production details: Made by transfecting the human embryo kidney cell line HEK293T with BRE-Luciferase plasmid, TK Renilla plasmid and pRetroSuper for puromycin resistance.
Parental cell line: HEK293T cell line
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Growth medium: Dulbecco's modified Eagle's medium (DMEM) containing 10% fetal calf serum (FCS)
Recommended controls: HEK293 parental line
Mycoplasma free: Yes
Biosafety level: 1
References:

Gori et al. 2021. eLife 8,10:e63545. PMID: 33416497

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