Anti-TagRFP (Chicken) “Brainbow” pAb

Contributors

Inventor Institute
Dawen Cai University of Michigan, USA
SKU: 155265
Product description: The TagRFP polycloncal antibody is one of the Brainbow collection. The antibody host species is a Chicken. TagRFP is a red fluorescent protein generated from Entacmaea quadricolor. The brainbow collection has been used in a multicolour labelling strategy for the fluorescent imaging of neuronal circuits and individual neurons in mice, drosophila and zebrafish and non-neuronal cells in mice. The Brainbow toolkit allows scientists to image highly complex tissue structures by relying on a stochastic method for making different expression ratio combinations of fluorescent proteins so that structures in proximity can be resolved.
Alternate name: Anti-TagRFP (Chicken)
Additional notes: To minimise background, the sample section thickness should be less than the working distance of the objective, generally <100 μm for high numerical aperture lenses.
Cat. #: 155265
Tool sub type: Primary antibody
Unit size: 100 μl
Research Fields: Neurobiology
Application: IF ; IP ; WB
Target: TagRFP
Host: Chicken
Class: Polyclonal
Primary citation: Cai et al. 2013. Nat Methods. 10(6):540-547. PMID: 23817127.
Target background: The TagRFP is a fluorescent proteins originated from sea anemone (Entacmaea quadricolor)
Format: Liquid
Storage buffer: Serum with 0.02% sodium azide
Storage conditions: Short-term storage: -20 °C (stable for ~1 year without noticeable degradation). Long-term storage: –80 °C (for >3 years).
Shipping conditions: Dry ice
References:

Roossien et al. 2019. Bioinformatics. 35(18):3544-3546. PMID: 30715234.

Chen et al. 2018. Mol Psychiatry. 23(7):1614-1625. PMID: 28761082.

Takesian et al. 2018. Nat Neurosci. 21(2):218-227. PMID: 29358666.

Roossien et al. 2017. Methods Mol Biol. 1642:211-228. PMID: 28815503.

Manent et al. 2017. Oncogene. 36(40):5576-5592. PMID: 28581519.

Chang et al. 2017. Nat Methods. 14(6):593-599. PMID: 28417997.

Zanca et al. 2017. Genes Dev. 31(12):1212-1227. PMID: 28724615.

Tillberg et al. 2016. Nat Biotechnol. 34(9):987-992. PMID: 27376584.

Cai et al. 2013. Nat Methods. 10(6):540-547. PMID: 23817127.

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