Cell line with unique integration events in the following genes, of which one is the most likely cause for estrogen independence: FLJ35036, LOC391679, FSTL5, TRPS1, RAP2C, SRD5AP1 and BNIP3L
| Inventor | Institute |
|---|---|
| Lambert Dorssers | Erasmus University Medical Center (Erasmus MC) |
| SKU: | 154556 |
|---|---|
| Product description: | Breast cancer is widely and effectively treated with endocrine treatment. However, in many cases the tumours will eventually progress into an estrogen-independent and therapy-resistant phenotype. Retroviral insertion mutagenesis was used to generate this cell line in order to elucidate the molecular mechanisms underlaying endocrine therapy failure. Using this method the main genes conferring estrogen independence in human breast cancer cells where identified. Genes located in the immediate pr… |
| Production details: | ZR-75-1 cells were infected with amphotropic, defective murine retrovirus and plated in medium containing 1uM of 4-hydroxy-tamoxifen. Within 5 weeks after the start of selection proliferating colonies were individually picked and expanded to stable cell lines |
| Cellosaurus ID: | CVCL_E4L6 |
| Parental cell line: | ZR-75-1 |
| Disease: | Cancer |
| Cat. #: | 154556 |
|---|---|
| Tool sub type: | Continuous |
| Unit size: | 1×10^6 cells / vial |
| Cancer types: | Breast cancer |
| Research Fields: | Cancer; Drug development |
| Organism: | Human |
| Tissue: | Breast |
| Model: | Cancer Model |
| Cancer Types In Detail: | Mammary ductal carcinoma |
| Format: | Frozen |
|---|---|
| Storage conditions: | Liquid Nitrogen |
| Shipping conditions: | Dry ice |
| Growth medium: | RPMI 1640 medium supplemented with 10% heat-inactivated bovine calf serum (RBCS) |
| Mycoplasma free: | Yes |
| Biosafety level: | 1 |
| References: |
van Agthoven et al. 2009. Breast Cancer Res Treat. 114(1):23-30. PMID: 18351453. |
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