Expresses skin cell markers; proinflammatory. Tissue is adult skin cells.
| Inventor | Institute |
|---|---|
| Aloysius Klingelhutz | The University of Iowa |
| SKU: | 154462 |
|---|---|
| Product description: | Expresses skin cell markers; proinflammatory. Tissue is adult skin cells. |
| Conditional: | No |
| Production details: | Cells were transduced with HPV-16 E6/E7 LXSN replication defective retrovirus and selected in G418 |
| Parental cell line: | Keratinocytes isolated from punch biopsies of the skin from the buttocks |
| Disease: | Cancer |
| Cat. #: | 154462 |
|---|---|
| Unit size: | 1×10^6 cells / vial |
| Research Fields: | Cancer |
| Organism: | Human |
| Tissue: | Skin |
| Growth properties: | Upregulation of telomerase and greatly extended lifespan (greater than 5 times the lifespan of untransduced keratinocytes) with no apparent crisis or slow down in growth. Despite upregulation of telomerase, E6/E7 transduced keratinocytes exhibited significant telomere shortening with passaging in culture |
| Format: | Frozen |
|---|---|
| Storage conditions: | Liquid Nitrogen |
| Shipping conditions: | Dry ice |
| Growth medium: | Keratinocyte Serum Free Medium (K-SFM) (Gibco™, 17005042) supplemented with 0.16 ng/mL EGF and 25 ug/mL BPE. Do not keep media for more than 1 month past adding supplements. Do not freeze and thaw media. Change media every other day. The cell line will not do well if they are left over the weekend without a media change or if they are left to grow to greater than 95% confluency. |
| Mycoplasma free: | Yes |
| Biosafety level: | 1 |
| References: |
Gourronc et al. 2010. Exp Dermatol. 19(3):279-88. PMID: 19558498. |
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