| Inventor |
|---|
| Pranab K Das |
| SKU: | 154113 |
|---|---|
| Product description: | Immortalised human keratinocytes are widely used for studies of skin biology and differentiation |
| Production details: | Keratinocyte cultures were establised from neonatal foreskin using standard methods. Keratinocytes were transfected with HPV16 genes E6 and E7 using the retroviral construct LXCN16E6E7. The E6E7 genes are under the control of the MMLV promoter-enhancer sequence. In additon the vector contained a geneticin resistance gene. The retroviral particles were produced by the packaging cell line PA317. The critical concentration of geneticin for transformed selection was 1mg/ml. |
| Parental cell line: | Human foreskin |
| Cat. #: | 154113 |
|---|---|
| Unit size: | 1×10^6 cells / vial |
| Research Fields: | Cell biology |
| Organism: | Human |
| Tissue: | Kidney |
| Model: | Immortalised Line |
| Growth properties: | Cells were routinely passaged 1:4 at confluency |
| Format: | Frozen |
|---|---|
| Shipping conditions: | Dry ice |
| Growth medium: | DulbeccoÄË?Â?Âs Modified Eagle Medium [High Glucose, 2mM L-glutamine] supplemented with 10% Fetal Calf Serum |
Please note we may take up to three days to respond to your enquiry.
CancerTools.org uses the contact information provided to respond to you about our research tools and service. For more information please review our privacy policy.