Anti-Protein Kinase C a [133]

Monoclonal antibody which specifically detects the a isoform of PKC.

Contributors

Institute
BioServ UK Ltd
Cat. #: 153646
Tool sub type: Primary antibody
Unit size: 100 ug
Research Fields: Cancer;Neurobiology
Application: IHC ; WB
Target: Protein Kinase C a
Reactivity: Human ; Mammals
Clone: 133
Host: Mouse
Class: Monoclonal
Alternate name: Protein kinase C alpha, PKC-A, PKC-alpha, PRKCA, PKCA, PRKACA
Product description: Monoclonal antibody which specifically detects the ? isoform of PKC. Background and Research Application The PKC family consists of fifteen isozymes in humans. Protein Kinase C alpha (PKC?) is involved in the regulation of cell proliferation during cell cycle progression. PKC-? is a 672 amino acid enzyme containing a single AGC-kinase C-terminal domain, a C2 domain, 2 phorbol-ester/DAGtype zinc fingers and a single protein kinase domain. Anti-PKC (133) recognizes the ? isoform of PKC and binds to a sequence at the C terminus of PKC? to detect its expression in vitro.
Conjugation: Unconjugated
Isotype: IgG2a
Molecular weight: 75 kDa
Immunogen: Recognizes sequence PQFVHPILQSAV at the C terminus at PKCa
Immunogen Uniprot ID: P17252
Myeloma used: Sp2/0-Ag14
Target background: Monoclonal antibody which specifically detects the a isoform of PKC. Background and Research Application The PKC family consists of fifteen isozymes in humans. Protein Kinase C alpha (PKCa) is involved in the regulation of cell proliferation during cell cycle progression. PKC-a is a 672 amino acid enzyme containing a single AGC-kinase C-terminal domain, a C2 domain, 2 phorbol-ester/DAGtype zinc fingers and a single protein kinase domain. Anti-PKC (133) recognizes the a isoform of PKC and binds to a sequence at the C terminus of PKCa to detect its expression in vitro.
Format: Liquid
Concentration: 1 mg/ml
Storage conditions: Store at -20° C frozen. Avoid repeated freeze / thaw cycles
Shipping conditions: Dry ice
References:

De Silva et al. 2017. Proc Natl Acad Sci U S A. 114(42):11211-11216. PMID: 28973921.

Wang et al. 2017. Neuron. 93(6):1359-1374.e6. PMID: 28262416.

Kim et al. 2016. Mol Neurodegener. 11:30. PMID: 27098079.

Sarria et al. 2016. J Neurosci. 36(10):2915-25. PMID: 26961947.

Sarria et al. 2015. Elife. 4:e06358. PMID: 25879270.

Donovan et al. 2012. Mol Pharmacol. 82(3):428-37. PMID: 22653970.

Long QT2 mutation on the Kv11.1 ion channel inhibits current activity by ablating a protein kinase Ca consensus site.

Cao et al. 2012. Proc Natl Acad Sci U S A. 109(20):7905-10. PMID: 22547806.

Regulators of G protein signaling RGS7 and RGS11 determine the onset of the light response in ON bipolar neurons.

Campo et al. 2010. Biochim Biophys Acta. 1802(11):1118-30. PMID: 20713153.

High-molecular weight hyaluronan reduced renal PKC activation in genetically diabetic mice.

Huang et al. 2009. Vis Neurosci. 26(5-6):443-52. PMID: 20003598.

Cell type-specific and light-dependent expression of Rab1 and Rab6 GTPases in mammalian retinas.

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