NUOC-1 cell line represents a novel ovarian cancer cell line derived from the ascites of a chemotherapy nave patient with a primary mixed endometrioid/ clear cell/ high grade serous ovarian cancer
| Inventor | Institute |
|---|---|
| Richard Edmondson | Northern Institute For Cancer Research, Newcastle University |
| SKU: | 153527 |
|---|---|
| Product description: | Epithelial ovarian cancer is diagnosed at late disease stage in more than 70% of patients. Ovarian cancer is the leading cause of gynaecological cancer mortality worldwide with a 5-year overall survival of 3039%. It has long been recognised by clinicians that ovarian cancer is a set of heterogeneous diseases. NUOC-1 cell line represents a novel ovarian cancer cell line derived from the ascites of a chemotherapy nave patient with a primary mixed endometrioid/ clear cell/ high grade serous ovarian cancer. NUOC-1 cells grow as an adherent monolayer. The cells are TP53 wildtype, positive for PTEN, HER2 and HER3 expression but negative for oestrogen, progesterone and androgen receptor expression. NUOC-1 cells are competent in homologous recombination and non-homologous end joining, but base excision repair defective. Karyotype analysis demonstrated a complex tetraploid karyotype |
| Alternate name: | HGSC, high grade mixed ovarian cancer, epithelial ovarian cancer |
| Conditional: | No |
| Production details: | Ascites were collected from patients undergoing surgery for ovarian cancer. 20 ml of ascites was added to 20 ml of warmed culture medium in T75 flask, and incubated at 37ÄÂ?°C, 5% CO2, 95% humidified air. The medium was aspirated and 13 ml of warmed fresh medium was replaced on day 3 to 5. The medium was replaced every 4 to 5 days until the cells approached confluence |
| Parental cell line: | Ascites of chemotherapy naive patient |
| Disease: | Cancer |
| Cat. #: | 153527 |
|---|---|
| Tool sub type: | Continuous |
| Unit size: | 1×10^6 cells / vial |
| Cancer types: | Ovarian cancer |
| Research Fields: | Cancer; Drug development |
| Organism: | Human |
| Tissue: | Ovary |
| Model: | Cancer Model |
| Growth properties: | Doubling time approximately 58 hours |
| Cancer Types In Detail: | High Grade Ovarian |
| Format: | Frozen |
|---|---|
| Storage conditions: | Liquid Nitrogen |
| Shipping conditions: | Dry ice |
| Growth medium: | RPMI 1640 medium supplemented with 20% FCS, 20 mM L-glutamine and 1% penicillin and streptomycin |
| Mycoplasma free: | Yes |
| Biosafety level: | 1 |
| References: |
McCormick et al. 2017. Oncotarget. 8(16):26832-26844. PMID: 28460465. Functional characterisation of a novel ovarian cancer cell line, NUOC-1. |
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