DOK Cell Line
Exclusive ToolA pre-malignant human oral keratinocyte cell line used to study oral carcinogenesis, malignant transformation, and tobacco carcinogen exposure. Available exclusively through CancerTools. Save 30% on this cell line this summer. Use code SUM26CELL at checkout.
Contributors
| Inventor | Institute |
|---|---|
| Sidney Chang | Cancer Research UK, London Research Institute: Lincoln's Inn Fields |
| SKU: | 153251 |
|---|---|
| Product description: | DOK cells show some stratification in confluent cultures and contain a keratin profile similar to the original dysplasia. Growth is stimulated by hydrocortisone and inhibited by cholera toxin. Expression of p53 is increased and a 12 bp in-frame deletion of the p53 gene could be identified. DOK cells are non-tumourigenic in athymic nude mice. |
| Alternate name: | dysplastic oral keratinocyte |
| Conditional: | Yes |
| Production details: | The dysplastic oral keratinocyte cell line DOK was isolated from a piece of dorsal tongue from a 57 year old man. After a squamous-cell carcinoma was removed from the patient, who had been a heavy smoker, the remaining dysplasia was removed subsequently and used to initiate primary cultures leading to the establishment of DOK. |
| Additional notes: | STR-PCR Data: Amelogenin: X,Y CSF1PO: 12 D13S317: 11,13 D16S539: 9,12 D5S818: 12 D7S820: 11,13 THO1: 6,8 TPOX: 10 vWA: 14,18 |
| Cellosaurus ID: | CVCL_1180 |
| Disease: | Cancer |
| Cat. #: | 153251 |
|---|---|
| Provenance and Ownership: | The DOK Cell Line is owned by Cancer Research UK. CancerTools supplies the DOK Cell Line under licence from Cancer Research UK and is the authorised source for its distribution. Sourcing DOK Cell Line directly from the IP owner ensures authenticity, provenance and compliance with all applicable licensing and research use requirements. |
| Unit size: | 1×10^6 cells / vial |
| Research Fields: | Cancer;Drug development |
| Organism: | Human |
| Tissue: | Tongue |
| Model: | Tumour cell line |
| Growth properties: | Adherent |
| Cancer Types In Detail: | Human Caucasian dysplastic oral keratinocyte |
| Format: | Frozen |
|---|---|
| Shipping conditions: | Dry ice |
| Growth medium: | DMEM + 2mM Glutamine + 5ug/ml Hydrocortisone + 10% Foetal Bovine Serum (FBS). |
| Subculture routine: | Split sub-confluent cultures (70-80%) 1:10 i.e. seeding at 2-4×10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C. |
| References: |
Burns et al. 1994. Br J Cancer. 70(4):591-595. PMID: 7917902. Chang et al. 1992. Int J Cancer. 52(6):896-902. PMID: 1459732. |
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