RPE1 FRT/TR Cell Line

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A human retinal pigment epithelial cell model used to study inducible gene expression, cell cycle regulation, and functional genomics. Available exclusively from CancerTools. Save 30% on this cell line this autumn. Use code ACA26CELL at checkout.

Contributors

Inventor Institute
Jonathon Pines University of Cambridge
SKU: 153242
Product description: RPE1 FRT/TR is an engineered RPE1 cell line generated by random integration of an FRT site and Tet repressor gene. Designed for use with the Flp-In™ system, it enables the generation of cell lines with tetracycline-inducible expression of genes of interest, providing a convenient platform for controlled gene expression studies. The line has been used to generate inducible cell models expressing APC15-IRES2-mRuby, APC15-3xFLAG and Cyclin B1-L45A-HA using a modified pcDNA5/FRT/TO vector.
Conditional: Yes
Conditional description: Tetracycline-inducible expression of genes of interest.
Production details: This tetracycline-inducible RPE1 cell line was created by random integration of an FRT site and a Tet repressor gene into retinal pigment epithelial 1 (RPE1) cells.
Additional notes: Selection markers: This cell line is resistant to Zeocin (FRT cassette) and Blasticidin (Tet repressor cassette). Resistance to Hygromycin and Puromycin is inherited from the parental RPE1 cell line. Flp-In™ integration: The originating laboratory replaced the hygromycin resistance cassette in the pcDNA5/FRT/TO vector with a neomycin resistance marker and used Geneticin (G418 analogue) for selection of successfully integrated clones. Culture recommendations: Use tetracycline-free serum when generating inducible cell lines to minimise background expression from tetracycline-responsive constructs.
Parental cell line: RPE1
Cat. #: 153242
Provenance and Ownership: The RPE1 FRT/TR Cell Line is owned by Cancer Research UK. CancerTools supplies the RPE1 FRT/TR Cell Line under licence from Cancer Research UK and is the authorised source for its distribution. Sourcing RPE1 FRT/TR Cell Line directly from the IP owner ensures authenticity, provenance and compliance with all applicable licensing and research use requirements.
Unit size: 1×10^6 cells / vial
Research Fields: Genetics
Organism: Human
Tissue: Eye
Growth properties: Adherent cell line
Initial handling information: Thaw cells rapidly and transfer into pre-warmed F12:DMEM (1:1) medium supplemented with 10% FCS. Allow cells to recover and establish normal growth before commencing transfection or Flp-In™ integration experiments.
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Growth medium: F12:DMEM (1:1) media supplemented with 10% FCS.
Mycoplasma free: Yes
Biosafety level: 1
References:

Mansfield et al. 2011. Nat Cell Biol. 13:1234-43. PMID: 21926987

Citations And Reviews

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