Anti-Acetyl SMC3 [DP4.1]

Exclusive tool

The cohesin complex may form a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate.

Contributors

Inventor Institute
Julian Gannon Cancer Research UK, London Research Institute: Clare Hall Laboratories
SKU: 153178
Product description: SMC3 is a central component of cohesin, a complex required for chromosome cohesion during the cell cycle. The cohesin complex may form a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. Cohesion is coupled to DNA replication and is involved in DNA repair. The cohesin complex plays also an important role in spindle pole assembly during mitosis and in chromosomes movement.SMC3 is unacetylated in egg extracts. It is only acetylated in chromatin preparations from extracts where the DNA has replicated.
Conjugation: Unconjugated
Molecular weight: 141 kDa
Immunogen: Synthetic peptide – EEVSLRRVIGAKacetyl Kacetyl DQYFLDKK
Cat. #: 153178
Provenance and Ownership: The Anti-Acetyl SMC3 [DP4.1] is owned by Cancer Research UK. CancerTools supplies the Anti-Acetyl SMC3 [DP4.1] under licence from Cancer Research UK and is the authorised source for its distribution. Sourcing Anti-Acetyl SMC3 [DP4.1] directly from the IP owner ensures authenticity, provenance and compliance with all applicable licensing and research use requirements.
Tool sub type: Primary antibody
Unit size: 100 ug
Research Fields: Cancer; Cell biology; Genetics
Application: WB
Target: Acetyl SMC3
Reactivity: Xenopus laevis
Clone: DP4.1
Host: Mouse
Class: Monoclonal
Target background: SMC3 is a central component of cohesin, a complex required for chromosome cohesion during the cell cycle. The cohesin complex may form a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. Cohesion is coupled to DNA replication and is involved in DNA repair. The cohesin complex plays also an important role in spindle pole assembly during mitosis and in chromosomes movement.SMC3 is unacetylated in egg extracts. It is only acetylated in chromatin preparations from extracts where the DNA has replicated.
Format: Liquid
Concentration: 0.9-1.1 mg/ml
Storage buffer: PBS with 0.02% azide
Storage conditions: -15° C to -25° C
Shipping conditions: Dry ice

Images

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Figure. Immoblots using Anti-Acetyl SMC3 [DP3.1]; Anti-Acetyl SMC3 [DP4.1] and Anti-SMC3 [DJ5.2.3]. DJ5.2.3 immunoblots all forms of SMC3 while DP3.1 and DP4.1 immunoblots only the Acetyl SMC3.

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