T47D-STn Cell Line

The parental T47D cell line was established from the pleural effusion of a ductal carcinoma of the breast. The tumour cell line, T47D-STn, was created as a breast cancer model…

Contributors

Inventor Institute
Joy Burchell ; Joyce Taylor-Papadimitriou King's College London
Cat. #: 153170
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Organism: Human
Tissue: Breast
Gender: Male
Product description: The parental T47D cell line was established from the pleural effusion of a ductal carcinoma of the breast. The tumour cell line, T47D-STn, was created as a breast cancer model which expresses the Sialyl-Tn, STn, antigen.T47D-STn cells overexpress the enzymes C2GnT1, a beta-1,3galactosyl-O-glycosyl-glycoprotein and glycosyltransferase, ST6GalNAc-I. ST6GalNAc-I which is found throughout the Golgi, can catalyse the transfer of sialic acid to N-acetylgalactosamine and is predominately limited to the gastrointestinal tract in normal adult tissues. Expression of ST6GalNAc-I in human cells promotes formation of the STn antigen which is a saccharide structure (N-acetylgalactosamine) linked to serine or threonine by a glycosidic bond. STn antigens are not usually found on healthy cell surfaces but often on human breast cancer cells.Together with wild-type T47D and T47D-E2J these cell lines represent a panel of isogenic cells with different O-linked glycosylation patterns.
Gender: Male
CRISPR: Yes
Conditional: No
Parental cell line: T47D
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Growth medium: DMEM 10% FCS, 500 ?g/ml G418, 300 ?g/ml hygromycin
Mycoplasma free: Yes
Biosafety level: 1
References:

Sewell et al. 2006. J Biol Chem. 281(6):3586-94. PMID: 16319059.

The ST6GalNAc-I sialyltransferase localizes throughout the Golgi and is responsible for the synthesis of the tumor-associated sialyl-Tn O-glycan in human breast cancer.

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