Anti-Lewis y/b hapten [NCRC37]

The antibody, NCRC37, is a pan-reactive, anti-colorectal tumour antibody and binds strongly to coloretal tumour cells and to post mortem cryopreserved tissues of brain, spleen, heat, lung, kidney, testes, oesophagus or stomach.

Contributors

Inventor Institute
Lindy Durrant University of Nottingham
Cat. #: 152939
Tool sub type: Primary antibody
Unit size: 100 ug
Research Fields: Immunology;Tissue-specific biology
Application: ELISA ; FACS ; IHC ; Fn
Target: Sialyltetraosylceramiden antigen on colorectal tumor cell surface
Reactivity: Human
Clone: NCRC37
Host: Mouse
Class: Monoclonal
Product description: Blood group related antigens are a group of carbohydrate determinants present on both glycoproteins and glycolipids. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Lewis- y/b is a cell surface carbohydrate blood group antigen expressed at very low levels in normal tissue, but often found to be over-expressed on breast, lung, colon and ovarian cancer cells. Lewis-y antigen, a difucosylatedtetrasaccharide, is found on type 2 blood group oligosaccharides. The antibody, NCRC37, is a pan-reactive, anti-colorectal tumour antibody and binds strongly to coloretal tumour cells and to post mortem cryopreserved tissues of brain, spleen, heat, lung, kidney, testes, oesophagus or stomach. Lewis y antigen has been evaluated as a clinical marker for the diagnosis and prognosis of cholangiocarcinoma, hepatocellular carcinoma and breast cancer.
Conjugation: Unconjugated
Isotype: IgG1
Immunogen: Tissue cultured colorectal tumor cell lines (C170, C146, Colo205, and JW)
Myeloma used: NS0
Target background: Blood group related antigens are a group of carbohydrate determinants present on both glycoproteins and glycolipids. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Lewis- y/b is a cell surface carbohydrate blood group antigen expressed at very low levels in normal tissue, but often found to be over-expressed on breast, lung, colon and ovarian cancer cells. Lewis-y antigen, a difucosylatedtetrasaccharide, is found on type 2 blood group oligosaccharides. The antibody, NCRC37, is a pan-reactive, anti-colorectal tumour antibody and binds strongly to coloretal tumour cells and to post mortem cryopreserved tissues of brain, spleen, heat, lung, kidney, testes, oesophagus or stomach. Lewis y antigen has been evaluated as a clinical marker for the diagnosis and prognosis of cholangiocarcinoma, hepatocellular carcinoma and breast cancer.
Format: Liquid
Concentration: 1.14 mg/ml
Storage buffer: PBS with 0.02% azide
Storage conditions: -15° C to -25° C
Shipping conditions: Dry ice
References:

Durrant et al. 2006. Cancer Res. 66(11):5901-9. PMID: 16740730.

A new anticancer glycolipid monoclonal antibody, SC104, which directly induces tumor cell apoptosis.

Durrant et al. 1993. Hybridoma. 12(6):647-60. PMID: 7507082.

Development of second generation monoclonal antibodies recognising Lewisy/b antigen by anti-idiotypic immunisation.

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