BICR 3 Cell Line

Adherent keratinocyte cell line derived from a squamous cell carcinoma of the alveolus of a Caucasian female. Keratin and involucrin markers present.

Contributors

Inventor Institute
Eric Kenneth Parkinson Cancer Research UK, Glasgow: The Beatson Institute
SKU: 152845
Product description: Adherent keratinocyte cell line derived from a squamous cell carcinoma of the alveolus of a Caucasian female. Keratin and involucrin markers present.
Alternate name: BICR-3; BICR3; Beatson Institute for Cancer Research 3
Conditional: Yes
Additional notes: STR-PCR Data: Amelogenin: X, X CSF1PO: 11,12 D13S317: 8,13 D16S539: 11 D5S818: 11,12 D7S820: 10,13 THO1: 6,7 TPOX: 8 vWA: 14,19
Cellosaurus ID: CVCL_2311
Receptors of note: Epidermal Growth Factor Receptor (EGFR)
Disease: Cancer
Cat. #: 152845
Provenance and Ownership: The BICR 3 Cell Line is owned by Cancer Research UK. CancerTools supplies the BICR 3 Cell Line under licence from Cancer Research UK and is the authorised source for its distribution. Sourcing BICR 3 Cell Line directly from the IP owner ensures authenticity, provenance and compliance with all applicable licensing and research use requirements.
Unit size: 1×10^6 cells / vial
Cancer types: Lung cancer
Research Fields: Apoptosis and autophagy;Cancer;Drug development;Genetics
Organism: Human
Tissue: Alveolar ridge
Model: Tumour line
Growth properties: Adherent
Cancer Types In Detail: Squamous cell carcinoma
Format: Frozen
Shipping conditions: Dry ice
Growth medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 0.4 micrograms/ml Hydrocortisone.
Subculture routine: Split sub-confluent cultures (70-80%) 1:2 using 0.05% trypsin/EDTA; 8% CO₂; 37°C. Suggested seeding density 4 x 10,000 cells/cm². A feeder layer of lethally-irradiated or mitomycin C-treated 3T3 feeder cells can be used to culture this cell line (however, this is not essential). Use of conditioned medium at a ratio of 1:1 with fresh medium can improve cell condition.
References:

Edington et al. 1995. Mol Carcinog. 13(4):254-265. PMID: 7646764.

Images

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Characteristics of the BICR human head and neck squamous cell carcinoma (HNSCC) cell line panel, including tissue of origin, tumour (T), node (N), and metastasis (M) stage, and TP53 mutation status.
BICR 3 Cell Line. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC).

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